Chromogranin A ELISA 试剂盒
Quick Overview for Chromogranin A ELISA 试剂盒 (ABIN2648557)
抗原
See all Chromogranin A (CHGA) ELISA试剂盒适用
检测方法
应用范围
样品类型
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灵敏度
- 2 ng/ml
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产品特性
- Human Chromogranin A ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Chromogranin A levels in EDTA-plasma and serum samples. This assay exclusively measures human Chromogranin A without the high dose "hook" effect up to 1,000,000 ng/mL.
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Chromogranin A (CHGA) ELISA Kit
CHGA 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Chromogranin A (CHGA) ELISA KitCHGA 适用: 人 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum, Urine
Chromogranin A (CHGA) ELISA KitCHGA 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Chromogranin A (CHGA) ELISA KitCHGA 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chromogranin A (CHGA) ELISA KitCHGA 适用: 小鼠 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Plasma, Serum, Tissue Homogenate
Chromogranin A (CHGA) ELISA KitCHGA 适用: 大鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum
Chromogranin A (CHGA) ELISA KitCHGA 适用: 人 Colorimetric Sandwich ELISA 62.5 ng/mL - 4000 ng/mL Cell Culture Supernatant, Cerebrospinal Fluid, Plasma, Serum, Urine
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应用备注
- Optimal working dilution should be determined by the investigator.
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样本量
- 15 μL
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实验时间
- 2.5 h
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实验流程
- Human Chromogranin A ELISA Assay Kit is designed, developed and produced for the quantitative measurement of human chromogranin A in EDTA-plasma or serum sample. The assay utilizes the two-site "sandwich" technique with two selected antibodies that bind to different epitopes of human Chromogranin A. Assay standards, controls and patient samples are added directly to wells of microplate that is coated with a polyclonal chromogranin A antibody. After the first incubation period, the antibody on the wall of microtiter well captures human chromogranin A in the sample and unbound proteins in each microtiter well is washed away. Then a horseradish peroxidase (HRP) labeled monoclonal anti-human chromogranin A antibody is added to each microtiter well and a "sandwich" of "monoclonal antibody - human chromogranin A - polyclonal antibody" is formed. The unbound monoclonal antibody is removed in the subsequent washing step. For the detection of this immunocomplex, the well is incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to the chromogranin A on the wall of the microtiter well is directly proportional to the amount of chromogranin A in the sample. A standard curve is generated by plotting the absorbance versus the respective human chromogranin A concentration for each standard with a 4 parameter curve fit. The concentration of human chromogranin A in test samples is determined directly from this standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C
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- Chromogranin A (CHGA)
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别名
- Chromogranin A
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途径
- Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling
抗原 See all Chromogranin A (CHGA) ELISA试剂盒
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