S100A8/A9 Complex (Calprotectin) ELISA 试剂盒
Quick Overview for S100A8/A9 Complex (Calprotectin) ELISA 试剂盒 (ABIN2648553)
抗原
See all S100A8/A9 Complex (Calprotectin) (S100A8/A9) ELISA试剂盒适用
检测方法
应用范围
样品类型
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灵敏度
- 5 ng/ml
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产品特性
- This Calprotectin ELISA Assay kit is intended for use in the quantitative determination of human calprotectin (neutrophil cytoplasmic protein S100A8/A9, MRP 8/14) levels in stool samples via Enzyme-linked immunosorbent assay. The Calprotectin ELISA Assay kit is for research use only and should not be used in diagnostic procedures.
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Calprotectin (S100A8/A9) ELISA Kit
S100A8/A9 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Calprotectin (S100A8/A9) ELISA KitS100A8/A9 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Calprotectin (S100A8/A9) ELISA KitS100A8/A9 适用: 绵羊 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Calprotectin (S100A8/A9) ELISA KitS100A8/A9 适用: 小鸡 Colorimetric Competition ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Calprotectin (S100A8/A9) ELISA KitS100A8/A9 适用: 犬 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Calprotectin (S100A8/A9) ELISA KitS100A8/A9 适用: Pig Colorimetric Competition ELISA 0.312 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Calprotectin (S100A8/A9) ELISA KitS100A8/A9 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Calprotectin (S100A8/A9) ELISA KitS100A8/A9 适用: 犬 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum
Calprotectin (S100A8/A9) ELISA KitS100A8/A9 适用: 人 Colorimetric Sandwich ELISA 0 μg/mL - 2000 μg/mL Fecal
Calprotectin (S100A8/A9) ELISA KitS100A8/A9 适用: 人 Colorimetric Sandwich ELISA 0.078-5 ng/mL Cell Culture Cells, Cerebrospinal Fluid, Milk, Plasma, Saliva, Serum, Urine
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应用备注
- Optimal working dilution should be determined by the investigator.
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样本量
- 50 mg
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实验时间
- 2 h
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实验流程
- Assay standards, controls and patient samples are added directly to wells of a microtiter plate that is coated with antibody to calprotectin. After a short incubation period, the plate is washed and horseradish peroxidase (HRP) conjugated human calprotectin specific monoclonal antibody is added to each well. After the second incubation period, a "sandwich" of solid-phase antibody - human calprotectin - HRP conjugated monoclonal antibody" is formed. The unbound monoclonal antibodies and buffer matrix are removed in the subsequent washing step. For the detection of this immunocomplex, the well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to the wall of each microtiter well is directly proportional to the amount of human calprotectin in the test sample. A standard curve is generated by plotting the absorbance versus the respective human calprotectin concentration for each standard on a point-to-point or 4-parameter curve fitting. The concentration of fecal human calprotectin in test samples is determined directly from this standard curve of the Calprotectin ELISA Assay.
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限制
- 仅限研究用
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储存条件
- 4 °C
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- S100A8/A9 Complex (Calprotectin) (S100A8/A9) (Calprotectin (S100A8/A9))
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别名
- Calprotectin
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途径
- S100 Proteins
抗原 See all S100A8/A9 Complex (Calprotectin) (S100A8/A9) ELISA试剂盒
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