CD86 ELISA 试剂盒
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Quick Overview for CD86 ELISA 试剂盒 (ABIN2648551)
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See all CD86 ELISA试剂盒适用
检测方法
应用范围
样品类型
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原理
- Human CD86 ELISA is to be used for the in-vitro quantitative determination CD86 in human serum, plasma, buffered solutions or cell culture medium. The assay will recognize both natural and recombinant human CD86.
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Analytical Method
- Quantitative
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灵敏度
- 0.6 U/ml
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产品特性
- Human CD86 ELISA Assay kit is for Research Use Only.
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CD86 (CD86) ELISA Kit
CD86 适用: 大鼠 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
CD86 (CD86) ELISA KitCD86 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
CD86 (CD86) ELISA KitCD86 适用: Cow Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
CD86 (CD86) ELISA KitCD86 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
CD86 (CD86) ELISA KitCD86 适用: 大鼠 AA 29-250 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Serum
CD86 (CD86) ELISA KitCD86 适用: 小鼠 AA 26-245 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Serum
CD86 (CD86) ELISA KitCD86 适用: 大鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Serum
CD86 (CD86) ELISA KitCD86 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Serum
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应用备注
- Optimal working dilution should be determined by the investigator.
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样本量
- 100 μL
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实验时间
- 2 - 3 h
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板类型
- Pre-coated
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实验流程
- The CD86 Kit is a solid phase sandwich Enzyme Linked-Immuno- Sorbent Assay (ELISA). A monoclonal antibody specific for CD86 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD86 concentrations and unknowns are pipetted into these wells. During the first incubation, the CD86 antigen and a biotinylated monoclonal antibody specific for CD86 are simultaneously incubated. After washing, the enzyme (streptavidin-peroxydase) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CD86 present in the samples.
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限制
- 仅限研究用
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储存条件
- 4 °C
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- CD86
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别名
- CD86
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途径
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
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