Thrombomodulin ELISA 试剂盒
Quick Overview for Thrombomodulin ELISA 试剂盒 (ABIN2648546)
抗原
See all Thrombomodulin (THBD) ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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原理
- sCD141 ELISA kit is a solid phase sandwich ELISA for the in-vitro qualitative and quantitative determination of sCD141 in supernatants, buffered solutions or serum and plasma samples. This assay will recognise both natural and recombinant human sCD141.
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Analytical Method
- Qualitative and Quantitative
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灵敏度
- 0.31 ng/ml
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产品特性
- Human sCD141 ELISA Assay kit is for Research Use Only.
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Thrombomodulin (THBD) ELISA Kit
THBD 适用: 人 Colorimetric Sandwich ELISA 46.88 pg/mL - 3000 pg/mL Plasma
Thrombomodulin (THBD) ELISA KitTHBD 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma
Thrombomodulin (THBD) ELISA KitTHBD 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma, Serum
Thrombomodulin (THBD) ELISA KitTHBD 适用: 犬 Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Plasma
Thrombomodulin (THBD) ELISA KitTHBD 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma
Thrombomodulin (THBD) ELISA KitTHBD 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Thrombomodulin (THBD) ELISA KitTHBD 适用: 犬 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Thrombomodulin (THBD) ELISA KitTHBD 适用: 大鼠 Colorimetric Sandwich ELISA 93.75 pg/mL - 6000 pg/mL Plasma, Serum, Tissue Homogenate
Thrombomodulin (THBD) ELISA KitTHBD 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Thrombomodulin (THBD) ELISA KitTHBD 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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样本量
- 100 μL
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实验时间
- 1 - 2 h
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板类型
- Pre-coated
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实验流程
- A capture Antibody highly specific for CD141 has been coated to the wells of the microtitre strip plate provided during manufacture. Binding of CD141 samples and known standards to the capture antibodies and subsequent binding of the biotinylated anti-CD141 secondary antibody to the analyte is completed during the same incubation period. Any excess unbound analyte and secondary antibody is removed. The HRP conjugate solution is then added to every well including the zero wells, following incubation excess conjugate is removed by careful washing. A chromogen substrate is added to the wells resulting in the progressive development of a blue coloured complex with the conjugate. The colour development is then stopped by the addition of acid turning the resultant final product yellow. The intensity of the produced coloured complex is directly proportional to the concentration of CD141 present in the samples and standards. The absorbance of the colour complex is then measured and the generated OD values for each standard are plotted against expected concentration forming a standard curve. This standard curve can then be used to accurately determine the concentration of CD141 in any sample tested.
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限制
- 仅限研究用
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储存条件
- 4 °C
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- Thrombomodulin (THBD)
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别名
- CD141 / THROMBOMODULIN
抗原 See all Thrombomodulin (THBD) ELISA试剂盒
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