CD25 ELISA 试剂盒
Quick Overview for CD25 ELISA 试剂盒 (ABIN2648538)
抗原
See all CD25 (IL2RA) ELISA试剂盒适用
检测方法
实验类型
应用范围
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原理
- sCD25 ELISA kit is a solid phase sandwich ELISA for the in-vitro qualitative and quantitative determination of sCD25 in supernatants, buffered solutions or serum and plasma samples. This assay will recognise both natural and recombinant human sCD25.
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Analytical Method
- Qualitative and Quantitative
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产品特性
- Human sCD25 ELISA Assay kit is for Research Use Only.
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Interleukin 2 Receptor, alpha (IL2RA) ELISA Kit
IL2RA 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 2 Receptor, alpha (IL2RA) ELISA KitIL2RA 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 2 Receptor, alpha (IL2RA) ELISA KitIL2RA 适用: 小鼠 Colorimetric Sandwich ELISA 78.13 pg/mL - 5000 pg/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 2 Receptor, alpha (IL2RA) ELISA KitIL2RA 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 2 Receptor, alpha (IL2RA) ELISA KitIL2RA 适用: Cow Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Plasma, Serum
Interleukin 2 Receptor, alpha (IL2RA) ELISA KitIL2RA 适用: 小鼠 AA 22-236 Colorimetric Sandwich ELISA 78-5000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Interleukin 2 Receptor, alpha (IL2RA) ELISA KitIL2RA 适用: 人 AA 22-213 Colorimetric Sandwich ELISA 78-5000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Interleukin 2 Receptor, alpha (IL2RA) ELISA KitIL2RA 适用: 人 Soluble Colorimetric Sandwich ELISA 78.13-5000 pg/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 2 Receptor, alpha (IL2RA) ELISA KitIL2RA 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Pre-coated
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实验流程
- A capture Antibody highly specific for CD25 has been coated to the wells of the microtitre strip plate provided during manufacture. Binding of CD25 samples and known standards to the capture antibodies and subsequent binding of the biotinylated anti-CD25 secondary antibody to the analyte is completed during the same incubation period. Any excess unbound analyte and secondary antibody is removed. The HRP conjugate solution is then added to every well including the zero wells, following incubation excess conjugate is removed by careful washing. A chromogen substrate is added to the wells resulting in the progressive development of a blue coloured complex with the conjugate. The colour development is then stopped by the addition of acid turning the resultant final product yellow. The intensity of the produced coloured complex is directly proportional to the concentration of CD25 present in the samples and standards. The absorbance of the colour complex is then measured and the generated OD values for each standard are plotted against expected concentration forming a standard curve. This standard curve can then be used to accurately determine the concentration of CD25 in any sample tested.
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限制
- 仅限研究用
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储存条件
- 4 °C
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- CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
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别名
- CD25 / IL-2R
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途径
- JAK/STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation
抗原 See all CD25 (IL2RA) ELISA试剂盒
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