Biotin ELISA 试剂盒
This 化学剂 Biotin ELISA Kit is a Colorimetric ELISA Kit designed to quantify 化学剂 Biotin.
Quick Overview for Biotin ELISA 试剂盒 (ABIN2648517)
抗原
See all Biotin products
Biotin
适用
化学剂
检测方法
Colorimetric
应用范围
ELISA
样品类型
Food
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灵敏度
- 0.5 ng/mL
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产品特性
- Biotin serves as the prosthetic group of enzymes, which catalyze carboxylations in the organism. For this purpose, biotin is bound via its carboxy group to lysin residues of carboxylases, and the transfer of carbon dioxide takes place after its attachment to a nitrogen atom of biotin, forming the so-called active carbon dioxide.
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Biotin ELISA Kit
适用: Various Species Colorimetric Competition ELISA 32 pg/mL - 20000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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实验时间
- 1.5 h
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板类型
- Pre-coated
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实验流程
- Biotin (Vitamin H) quantitative test is based on the principle of the enzyme linked immu-nosorbent assay. Avidin, which shows a high affinity to biotin, is bound on the surface of a microtiter plate. Biotin containing samples or standards and a biotin-alkaline phosphatase conjugate are given into the wells of the microtiter plate. Enzyme labelled and free biotin compete for the binding sites. After one hour incubation at room temperature, the wells are washed with diluted washing solution to remove un-bound material. A substrate solution is added and incubated for 30 minutes, resulting in the develop-ment of a yellow colour. The colour development is inhibited by the addition of a stop solution. The yellow colour is measured photometrically at 405 nm. The concentra-tion of biotin is indirectly proportional to the colour inten-sity of the Biotin ELISA Assay test sample.
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限制
- 仅限研究用
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储存条件
- 4 °C
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- Biotin
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物质类
- Chemical
抗原 See all Biotin products
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