ACTH ELISA 试剂盒
Quick Overview for ACTH ELISA 试剂盒 (ABIN2648478)
抗原
See all ACTH ELISA试剂盒适用
检测方法
应用范围
样品类型
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灵敏度
- .4 pg/ml
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产品特性
- Human Adrenocorticotrophic Hormone (ACTH) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Adrenocorticotrophic Hormone (ACTH) levels in plasma. Human Adrenocorticotrophic Hormone (ACTH) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.
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Adrenocorticotropic hormone (ACTH) ELISA Kit
ACTH 适用: 小鼠 Colorimetric Competition ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Adrenocorticotropic hormone (ACTH) ELISA KitACTH 适用: 人 Colorimetric Competition ELISA 1.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Adrenocorticotropic hormone (ACTH) ELISA KitACTH 适用: 小鼠 Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Plasma, Serum
Adrenocorticotropic hormone (ACTH) ELISA KitACTH 适用: 大鼠 Colorimetric Competition ELISA 1.23 pg/mL - 100 pg/mL Plasma, Serum
Adrenocorticotropic hormone (ACTH) ELISA KitACTH 适用: 小鼠 Colorimetric Sandwich ELISA 18.75 pg/mL - 1200 pg/mL Plasma, Serum
Adrenocorticotropic hormone (ACTH) ELISA KitACTH 适用: 大鼠 Colorimetric Competition ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Adrenocorticotropic hormone (ACTH) ELISA KitACTH 适用: 大鼠 Colorimetric Competition ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Adrenocorticotropic hormone (ACTH) ELISA KitACTH 适用: 小鼠 Colorimetric Competition ELISA 12.5 pg/mL - 800 pg/mL Plasma, Serum, Tissue Homogenate
Adrenocorticotropic hormone (ACTH) ELISA KitACTH 适用: Various Species Colorimetric Competition ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Adrenocorticotropic hormone (ACTH) ELISA KitACTH 适用: Pig Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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样本量
- 200 μL
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实验时间
- 2.5 h
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实验流程
- Human Adrenocorticotrophic Hormone (ACTH) ELISA Assay Kit is designed, developed and produced for the quantitative measurement of human ACTH in EDTA-plasma sample. The assay utilizes the two-site "sandwich" technique with selected antibodies that bind to N-terminal and C-terminal epitopes of ACTH. Assay standards, controls and patient samples are added directly to wells of a microtiter plate that is coated with antibody to the N-terminal of human ACTH. Immediately, a horseradish peroxidase (HRP) conjugated anti C-terminal of human ACTH antibody is added to each well. After the first incubation period, a "sandwich" of solid-phase monoclonal antibody - human ACTH - HRP conjugated antibody" is formed. The unbound antibodies and buffer matrix are removed in the subsequent washing step. For the detection of this immunocomplex, the well is then incubated with a substrate solution in a timed reaction and the absorbances are then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to the wall of each microtiter well is directly proportional to the amount of human ACTH in the test sample. A standard curve is generated by plotting the absorbance versus the respective human ACTH concentration for each standard on a point-to-point or 4-parameter curve fitting. The concentration of human ACTH in test samples is determined directly from this standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C
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- ACTH (Adrenocorticotropic hormone (ACTH))
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物质类
- Hormone
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途径
- Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity
抗原 See all ACTH ELISA试剂盒
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