Hepcidin ELISA 试剂盒
Quick Overview for Hepcidin ELISA 试剂盒 (ABIN2534450)
抗原
See all Hepcidin (HAMP) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.156 ng/mL
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原理
- Mouse Hepcidin (HAMP) ELISA Kit
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Analytical Method
- Quantitative
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灵敏度
- < 0.06 ng/mL
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组件
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The kit components listed are for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.
- Pre-coated 96-Well Microplate
- Standard
- Standard Diluent Buffer
- Wash Buffer
- Detection Reagent A
- Detection Reagent B
- Diluent A
- Diluent B
- TMB Substrate
- Stop Solution
- Plate Sealer
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试剂未包括
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- 37 °C incubator
- Multi and single channel pipettes and sterile pipette tips
- Squirt bottle or automated microplate washer
- 1.5 mL tubes
- Distilled water
- Absorbent filter papers
- 100 mL and 1 liter graduated cylinders
- Microplate reader (wavelength: 450 nm)
- ELISA Shaker
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Hepcidin Antimicrobial Peptide (HAMP) ELISA Kit
HAMP 适用: 人 Colorimetric Competition ELISA 320 pg/mL - 200000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Hepcidin Antimicrobial Peptide (HAMP) ELISA KitHAMP 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Hepcidin Antimicrobial Peptide (HAMP) ELISA KitHAMP 适用: 小鼠 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Cell Culture Supernatant, Plasma, Serum
Hepcidin Antimicrobial Peptide (HAMP) ELISA KitHAMP 适用: 小鼠 Colorimetric Competition ELISA 1.23 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Hepcidin Antimicrobial Peptide (HAMP) ELISA KitHAMP 适用: 大鼠 Colorimetric Competition ELISA 160 pg/mL - 100000 pg/mL Biological Fluids, Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Hepcidin Antimicrobial Peptide (HAMP) ELISA KitHAMP 适用: Pig Colorimetric Competition ELISA 740.74 pg/mL - 60000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Hepcidin Antimicrobial Peptide (HAMP) ELISA KitHAMP 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Urine
Hepcidin Antimicrobial Peptide (HAMP) ELISA KitHAMP 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Hepcidin Antimicrobial Peptide (HAMP) ELISA KitHAMP 适用: 小鼠 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum, Tissue Homogenate
Hepcidin Antimicrobial Peptide (HAMP) ELISA KitHigh Sensitivity HAMP 适用: 大鼠 Colorimetric Sandwich ELISA 246.9 pg/mL - 20000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal dilutions/concentrations should be determined by the end user.
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说明
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The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5 % within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.
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样本量
- 50 µL
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板类型
- Pre-coated
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试剂准备
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This procedure is provided for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.
- 1) Standard: Prepare the standard with the recommended volume of Standard Diluent Buffer, to make the standard solution. Then use the Standard Diluent buffer to carry out serial dilutions of the standard solution, as instructed in the Protocol.
- 2) Wash Buffer: Dilute the concentrated Wash Buffer with distilled water, as instructed in the Protocol.
- 3) Detection Reagent Preparation: Calculate the total volume of working solution required. Dilute Detection Reagent A and Detection Reagent B with Diluent A and Diluent B, respectively, at 1:100.
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实验流程
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This procedure is provided for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.
- Equilibrate the kit components and samples to room temperature (18 - 25 °C) before use. It is recommended to plot a standard curve for each test.
- 1. Set standard, test sample and control (zero) wells on the pre-coated plate respectively, and then, record their positions. It is recommended to measure each standard and sample at least in duplicate.
- 2. Add 50 μL of each standard, control and sample into the appropriate wells.
- 3. Remove the cover and discard the liquid.
- 4. Immediately aliquot 50 μL of Detection Reagent A working solution. Seal the plate with a cover and incubate for 1 h at 37 °C.
- 5. Remove the cover and discard the solution. Wash the plate 3 times with 1X Wash Buffer.
- 6. Add 100 μL of Detection Reagent B working solution into each well, seal and incubate at 37 °C for 30 min.
- 7. Discard the solution and wash the plate 5 times with wash buffer as explained in previous step.
- 8. Aliquot 90 μL of TMB Substrate into each well. Seal the plate with a cover and incubate at 37 °C for 10-20 min. Avoid exposure to light. The incubation time is for reference use only, the optimal time should be determined by end user. Do not exceed 30 min.
- 9. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
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结果分析
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This assay is competitive, therefore there is an inverse correlation between Hepc25 concentration in the sample and the absorbance measured. Create a graph with the log of the standard concentration (y-axis) and average absorbance measured (x-axis). Apply a best fit trendline through the standard points. The Pro-Hepcidin (Pro-HAMP) concentration of the samples can be interpolated from the standard curve.
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实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, medium and high levels of Pro-Hepcidin (Pro-HAMP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, medium and high levels of Pro-Hepcidin (Pro-HAMP) were tested on 3 different plates, 8 replicates in each plate.
CV (%) = (Standard Deviation / mean) x 100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.
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有效期
- 6 months
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- Hepcidin (HAMP) (Hepcidin Antimicrobial Peptide (HAMP))
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别名
- Hepcidin
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途径
- Hormone Activity, Transition Metal Ion Homeostasis
抗原 See all Hepcidin (HAMP) ELISA试剂盒
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