GLP-1 ELISA 试剂盒
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- 抗原 See all GLP-1 ELISA试剂盒
- GLP-1 (Glucagon-like peptide 1 (GLP-1))
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适用
- 猕猴
- 检测方法
- Colorimetric
- 实验类型
- Competition ELISA
- 检测范围
- 12.35-1000 pg/M
- 最低检测浓度
- 12.35 pg/M
- 应用范围
- ELISA
- 原理
- This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific for Glucagon Like Peptide 1 (GLP1) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between Horseradish Peroxidase (HRP) labeled Glucagon Like Peptide 1 (GLP1) and unlabeled Glucagon Like Peptide 1 (GLP1) (Standards or samples) with the pre-coated antibody specific for Glucagon Like Peptide 1 (GLP1). After incubation the unbound conjugate is washed off. The amount of bound HRP conjugate is reverse proportional - the concentration of Glucagon Like Peptide 1 (GLP1) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional - the concentration of Glucagon Like Peptide 1 (GLP1) in the sample.
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
- 特异性
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This assay has high sensitivity and excellent specificity for detection of Glucagon Like Peptide 1 (GLP1)
No significant cross-reactivity or interference between Glucagon Like Peptide 1 (GLP1) and analogues was observed. - 灵敏度
- The minimum detectable dose of this kit is typically less than 4.77 pg/m
- Top Product
- Discover our top product GLP-1 ELISA Kit
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Glucagon-like peptide 1 (GLP-1) ELISA Kit
GLP-1 适用: 人, 小鼠, 大鼠, 犬, Cow, 兔 Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Plasma, Serum
Glucagon-like peptide 1 (GLP-1) ELISA KitGLP-1 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 pg/mL - 100 pg/mL Cell Culture Supernatant, Plasma, Serum
Glucagon-like peptide 1 (GLP-1) ELISA KitGLP-1 适用: 小鼠 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Glucagon-like peptide 1 (GLP-1) ELISA KitGLP-1 适用: 人 Colorimetric Sandwich ELISA 1.56 pg/mL - 100 pg/mL Cell Culture Supernatant, Plasma, Serum
Glucagon-like peptide 1 (GLP-1) ELISA KitGLP-1 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Glucagon-like peptide 1 (GLP-1) ELISA KitGLP-1 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Glucagon-like peptide 1 (GLP-1) ELISA KitGLP-1 适用: 大鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Glucagon-like peptide 1 (GLP-1) ELISA KitGLP-1 适用: Pig Colorimetric Competition ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Glucagon-like peptide 1 (GLP-1) ELISA KitGLP-1 适用: 人 Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Glucagon-like peptide 1 (GLP-1) ELISA KitGLP-1 适用: Pig Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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The standard curve concentrations used for the ELISA's were 1000 pg/mL, 333.33 pg/mL, 111.11 pg/mL, 37.04 pg/mL, 12.35 pg/mL
- 实验时间
- 2.5 h
- 板类型
- Strips
- 实验流程
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1. Prepare all reagents, samples and standards,
2. Add 50 μL standard or sample to each well.
And then add 50 μL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37 °C,
3. Aspirate and wash 3 times,
4. Add 100 μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
5. Aspirate and wash 5 times,
6. Add 90 μL Substrate Solution. Incubate 15-25 minutes at 37 °C,
7. Add 50 μL Stop Solution. Read at 450 nm immediately. - 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glucagon Like Peptide 1 (GLP1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glucagon Like Peptide 1 (GLP1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
- 限制
- 仅限研究用
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- 注意事项
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end. - 储存条件
- 4 °C
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- 抗原 See all GLP-1 ELISA试剂盒
- GLP-1 (Glucagon-like peptide 1 (GLP-1))
- 别名
- Glucagon Like Peptide 1 (GLP1) (GLP-1 产品)
- 别名
- GLP1 ELISA Kit, GLP2 ELISA Kit, GRPP ELISA Kit, GLP-1 ELISA Kit, Glu ELISA Kit, PPG ELISA Kit, glucagon ELISA Kit, GCG ELISA Kit, Gcg ELISA Kit
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