(10 references)-
- 适用
- Adenovirus
- 应用范围
- Purification (Purif)
- 品牌
- ViraBind™
- 样品类型
- Cell Extracts, Cell Samples
- 产品特性
- ViraBind™ Adenoviral Miniprep Kit does not involve ultracentrifugation, instead viruses are captured on spin column based on the unique properties of adenoviral capsid proteins. The entire procedure takes about 30 minutes. Each column is designed to purify viruses harvested from one T75 flask or 11 10-cm plate, and has a capacity of up to 1.0 x 10 VPs. ViraBind™ Adenovirus Miniprep Kit provides an efficient system for quick adenoviral purification with high recovery (>95 % ). The system may be adapted to purification of other viral types, such as retrovirus and lentivirus.
- 组件
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- ViraBind™ Columns and Collection tubes : Pack of 10 mini spin columns and 20 collection tubes.
- Loading Buffer : One 10 mL bottle.
- Wash Buffer : One 20 mL bottle. 2
- Elution Buffer : One 10 mL bottle of 25 mM Tris, pH 7.5, 2.5 mM Mg2Cl, 1 M NaCl.
- 试剂未包括
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- Recombinant adenovirus of interest
- HEK 293 cells and cell culture growth medium
- Cell culture centrifuge
- Glycerol
- Microcentrifuge
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Tumor Necrosis Factor alpha (TNF alpha) ELISA Kit
TNF alpha 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor alpha (TNF alpha) ELISA KitTNF alpha 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
High Mobility Group Box 1 (HMGB1) ELISA KitHMGB1 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum
Lipopolysaccharides (LPS) ELISA Kit适用: Various Species Colorimetric Competition ELISA 12.35 ng/mL - 1000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor alpha (TNF alpha) ELISA KitTNF alpha 适用: 人 AA 77-233 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Interleukin 6 (IL6) ELISA KitIL6 适用: 人 Colorimetric Sandwich ELISA 7.8-500 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA KitVCAM1 适用: 人 Colorimetric Sandwich ELISA 1.563-100 ng/mL Plasma, Serum, Tissue Homogenate
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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- Achieve the quality of CsCl procedures in <2 hours, without ultracentrifugation
- Recover >90% adenoviral yield
- 限制
- 仅限研究用
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- 注意事项
- Remember that you will be working with samples containing infectious virus. Follow the recommended NIH guidelines for all materials containing BSL-2 organims.
- 储存条件
- RT
- 储存方法
- Store all kit components at room temperature.
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: "Standard free droplet digital polymerase chain reaction as a new tool for the quality control of high-capacity adenoviral vectors in small-scale preparations." in: Human gene therapy methods, Vol. 26, Issue 1, pp. 25-34, (2015) (PubMed).
: "Inhibition of porcine reproductive and respiratory syndrome virus infection by recombinant adenovirus- and/or exosome-delivered the artificial microRNAs targeting sialoadhesin and CD163 receptors." in: Virology journal, Vol. 11, pp. 225, (2015) (PubMed).
: "Inhibition of porcine reproductive and respiratory syndrome virus replication with exosome-transferred artificial microRNA targeting the 3' untranslated region." in: Journal of virological methods, Vol. 223, pp. 61-8, (2015) (PubMed).
: "Laboratory-Scale Production of Replication-Deficient Adenovirus Vectored Vaccines." in: Methods in molecular biology (Clifton, N.J.), Vol. 1349, pp. 121-35, (2015) (PubMed).
: "TRIM21, a negative modulator of LFG in breast carcinoma MDA-MB-231 cells in vitro." in: International journal of oncology, Vol. 47, Issue 5, pp. 1634-46, (2015) (PubMed).
: "Overexpression of a DENND1A isoform produces a polycystic ovary syndrome theca phenotype." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, Issue 15, pp. E1519-27, (2014) (PubMed).
: "Additive inhibition of porcine reproductive and respiratory syndrome virus infection with the soluble sialoadhesin and CD163 receptors." in: Virus research, Vol. 179, pp. 85-92, (2014) (PubMed).
: "Mitochondrial glutathione transport is a key determinant of neuronal susceptibility to oxidative and nitrosative stress." in: The Journal of biological chemistry, Vol. 288, Issue 7, pp. 5091-101, (2013) (PubMed).
: "MicroRNA-195 regulates vascular smooth muscle cell phenotype and prevents neointimal formation." in: Cardiovascular research, Vol. 95, Issue 4, pp. 517-26, (2012) (PubMed).
: "Gbetagamma signaling promotes breast cancer cell migration and invasion." in: The Journal of pharmacology and experimental therapeutics, Vol. 333, Issue 2, pp. 393-403, (2010) (PubMed).
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: "Standard free droplet digital polymerase chain reaction as a new tool for the quality control of high-capacity adenoviral vectors in small-scale preparations." in: Human gene therapy methods, Vol. 26, Issue 1, pp. 25-34, (2015) (PubMed).
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- 背景
- Recombinant adenoviruses have tremendous potential in both research and therapeutic applications. There are numerous advantages in using an adenovirus to introduce genetic material into host cells. The permissive host cell range is very wide. The virus has been used to infect many mammalian cell types (both replicative and non-replicative) for high expression of the recombinant protein. Recombinant adenoviruses are especially useful for gene transfer and protein expression in cell lines that have low transfection efficiency with liposome. After entering cells, the virus remains epichromosomal (i.e. does not integrate into the host chromosome so does not activate or inactivate host genes). Recently, recombinant adenoviruses have been used to deliver RNAi into cells. HEK 293 cells or their variants are used as host cells for viral amplification. Recombinant 10 adenoviruses can be grown at high titer (10 VP (viral particles)/mL, which can be concentrated up to 13 10 VP/mL). The concentrated viral supernatant is subjected to CsCl ultracentrifugation to separate the viruses from the cellular proteins and media components. Following ultracentrifugation, CsCl is then removed by dialysis. The CsCl procedure is both tedious and time consuming (16-24 hrs).
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