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- 应用范围
- Purification (Purif)
- 产品特性
- Lipid Extraction Kit isolates total lipids by organic extraction, but circumvents the above disadvantages by extracting lipids to an upper organic phase (making it amenable to high throughput extraction) that is chloroform free. A crude lipid source such as serum or tissue culture cell pellet is resuspended in a proprietary alcohol. After adding a proprietary organic compound, the mixture is centrifuged to gravitationally separate the phases. The recovered upper organic phase is then dried and resuspended for downstream lipid analysis. Each kit provides sufficient reagents to isolate up to 50 preps based on a 100 μL sample size. Larger sample sizes may be used (see Table 1) yielding proportionally fewer preps per kit.
- 组件
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- Lipid Extraction Reagent A : One 25 mL amber glass bottle.
- Lipid Extraction Reagent B : One 75 mL amber glass bottle.
- Lipid Extraction Reagent C : One 25 mL bottle.
- 试剂未包括
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- Glass tubes, 15 mL conical tubes, or microcentrifuge tubes
- 10 μL to 1000 μL adjustable single channel micropipettes with disposable tips
- 50 μL to 1000 μL adjustable multichannel micropipette with disposable tips
- Multichannel micropipette reservoir
- Phosphate buffered saline (PBS)
- Tube vortexer
- Organic solvent (such as chloroform, butanol, or cyclohexane)
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Tumor Necrosis Factor alpha (TNF alpha) ELISA Kit
TNF alpha 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor alpha (TNF alpha) ELISA KitTNF alpha 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
High Mobility Group Box 1 (HMGB1) ELISA KitHMGB1 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum
Lipopolysaccharides (LPS) ELISA Kit适用: Various Species Colorimetric Competition ELISA 12.35 ng/mL - 1000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor alpha (TNF alpha) ELISA KitTNF alpha 适用: 人 AA 77-233 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Interleukin 6 (IL6) ELISA KitIL6 适用: 人 Colorimetric Sandwich ELISA 7.8-500 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA KitVCAM1 适用: 人 Colorimetric Sandwich ELISA 1.563-100 ng/mL Plasma, Serum, Tissue Homogenate
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 样品制备
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- Plasma: Collect blood with an anticoagulant such as citrate, EDTA, heparin, or oxalate and mix by inversion. Centrifuge the blood at 1000 x g at 4 °C for 10 minutes. Collect plasma supernatant without disturbing the white buffy layer. Samples should be extracted immediately or may be stored at -80 °C.
- Serum: Collect blood in a tube with no anticoagulant. Allow the blood to clot at room temperature for 30 minutes. Centrifuge at 2500 x g for 20 minutes. Remove the yellow serum supernatant without disturbing the white buffy layer. Samples should be extracted immediately or may be stored at -80 °C.
- Cultured Cells: Pellet 5-10 x 106 cells at 1000 x g for 5 minutes. Wash cells once with 1X PBS, and resuspend final cell pellet with 100 μL 1X PBS. Perform the extraction as described in the kit protocol below.
- Tissues: Carefully mince the tissue into small fragments with a scalpel/razor blade and weigh in a 50 mL conical tube. Add PBS to a final tissue concentration of 2 mg/mL. Homogenize the tissue at 4 °C. Perform the extraction from the whole tissue homogenate as described in the kit protocol below.
- 限制
- 仅限研究用
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- 储存条件
- RT
- 储存方法
- Store the entire kit at room temperature. To avoid possible leakage store bottles upright.
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: "Caffeic Acid Expands Anti-Tumor Effect of Metformin in Human Metastatic Cervical Carcinoma HTB-34 Cells: Implications of AMPK Activation and Impairment of Fatty Acids De Novo Biosynthesis." in: International journal of molecular sciences, Vol. 18, Issue 2, (2017) (PubMed).
: "Granulocyte/Macrophage Colony-stimulating Factor-dependent Dendritic Cells Restrain Lean Adipose Tissue Expansion." in: The Journal of biological chemistry, Vol. 290, Issue 23, pp. 14656-67, (2015) (PubMed).
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: "Caffeic Acid Expands Anti-Tumor Effect of Metformin in Human Metastatic Cervical Carcinoma HTB-34 Cells: Implications of AMPK Activation and Impairment of Fatty Acids De Novo Biosynthesis." in: International journal of molecular sciences, Vol. 18, Issue 2, (2017) (PubMed).
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- 背景
- Lipids are a diverse group of molecules that include monoglycerides, diglycerides, trigylcerides, fats, sterols, and others. Not only do lipids define and preserve cellular membrane integrity, but they are also involved in cellular processes such as membrane trafficking, signal transduction, apoptosis, and energy storage. Perturbation in the metabolism of lipids has been linked to many diseases such as cancer, diabetes, Alzheimer's disease, and coronary heart disease. In order to study lipids, they must often be extracted first from tissues or cultured cells. Traditionally, organic extraction by the Folch method (Ref. 1) has been preferred, but this method has several disadvantages. First, it extracts lipids to a bottom organic phase, forcing penetration of the upper protein-containing phase during purification and causing contamination of lipid samples. As a result, low purity lipid samples can hamper downstream lipid analysis by clogging instruments such as high pressure liquid chromatographs (HPLCs). In addition, the Folch method uses chloroform as the organic phase solvent. Long-term exposure to chloroform by inhalation has resulted in effects on the liver such as hepatitis and jaundice. Furthermore, chloroform has been demonstrated to be carcinogenic in animals, causing in an increase in kidney and liver tumors. In fact, the United States Environmental Protection Agency (EPA) has classified chloroform as a Group B2, probable human carcinogen.
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