Quick Overview for HDL Purification Kit (Ultracentrifugation Free) ()
应用范围
样品类型
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Key Features
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- Frequently cited in scientific publications with 300+ citations
- Validated inhouse for relevant applications
- Extensive validation report for IF provided by one of your peers
- Designed to detect RFP and its variants
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Key Features
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- Multiplex ELISA: Testing 5 Targets in 1 Run
- High performance COVID-19 antibody testing
- 99.6% specificity, 95.9% sensitivity
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Key Features
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- Recombinant human neutralizing antibody (hNAb) against SARS-CoV-2.
- Binds SARS-CoV-2 S proteins of lineages B.1.1.7 (alpha), B.1.351 (beta), P.1 (gamma), B.1.617.2 (delta), B.1.1.529 (omicron), B.1.429 (epsilon), B.1.525 (eta), and B.1.617.1 (kappa).
- Frequently used as reference in S-protein ELISAs and neutralization assays.
- Synergizes with other hNAbs: binds a highly conserved epitope, not interefering with the S-protein's ACE2 RBD.
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Key Features
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- Recombinant human neutralizing antibody (hNAb) against SARS-CoV-2.
- Binds SARS-CoV-2 S proteins of lineages B.1.1.7 (alpha), B.1.351 (beta), P.1 (gamma), B.1.429 (epsilon), and B.1.525 (eta).
- Frequently used as reference in S-protein ELISAs and neutralization assays.
- Synergizes with other hNAbs: binds a highly conserved epitope.
- No interference with the ACE2 binding site.
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Key Features
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- Dendra2 Antibody (ABIN361314) independently validated for Immunocytochemistry by Neurology Lab (INSERM).
- Publications cite use for Immunoblotting as well as Immunostaining.
- Dendra2 belongs to a new class of photoactivatable fluorescent proteins (PAFPs). PAFPs allow to photolable and track fusion proteins in vivo. Dendra2 Antibody (ABIN361314) can be used for control purposes in this setting.
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Key Features
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- Magnetic agarose concanavalin A beads for CUT&RUN and CUT&Tag assays.
- Superior handling compared to magnetic silica concanavalin A beads: easier washes and resuspension.
- Reduced risk for samples to dry out.
- Performance comparable to or better than magnetic silica concanavalin A beads.
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Key Features
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- Recombinant SARS-CoV-2 spike protein produced in HEK-293.
- Contains the E484K mutation (present in the novel lineage 501Y.S2 from South Africa) that can affect antibody recognition and enable SARS-CoV-2 immune escape.
- Contains the D614G mutation that renders SARS-CoV-2 infection and replication more efficient.
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Key Features
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- Recombinant SARS-CoV-2 spike protein RBD produced in HEK-293.
- Contains the spike protein N501Y mutation (present in the novel lineages B.1.1.7 and 501Y.S2) that has been associated with an increased transmission rate of SARS-CoV-2.
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Key Features
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- Recombinant SARS-CoV-2 spike protein RBD produced in HEK-293.
- Contains the spike protein N501Y mutation (present in the novel lineages B.1.1.7 and 501Y.S2) that has been associated with an increased transmission rate of SARS-CoV-2.
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Key Features
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- Simultaneous detection of Sp Cas9 binding and cleavage sites in genomic DNA.
- In situ assay leaves cells intact and reduces background.
- Unbiased detection of off-site effects because it is not based on a predictive algorithm.
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Key Features
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- Detection of SARS-CoV-2 with variety of different immunoassays, i.e. ELISA, Lateral Flow, as validated by numerous publications (see below).
- Nucleocapsid antibody used for diagnostic Covid19 testing as well as for drug discovery.
- Developed and manufactured in the U.S.
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Key Features
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- Has been shown to neutralize SARS-Cov-2 S protein RBD from lineages B.1.1.7, B.1.351, P.1, and B.1.429.
- Suitable as positive control in neutralization antibody screening.
- Humanized chimeric antibody consisting of human IgG1 constant domains and murine variable regions.
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Key Features
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- Recombinant trimeric S protein of the SARS-CoV-2 variant of concern B.1.617.
- Contains E154K, L452R, E484Q, D614G, and P681R mutations characteristic for the SARS-CoV-2 kappa variant (B.1.617.1).
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Key Features
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- Recombinant trimeric S protein of the SARS-CoV-2 variant of interest B.1.525.
- Contains Q52R, del 69-70, del 144, E484K, F888L, and Q677H mutations characteristic for the SARS-CoV-2 eta variant (B.1.525).
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Key Features
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- Recombinant trimeric S protein of the SARS-CoV-2 variant of interest B.1.429.
- Contains S13I, W152C, L452R, and D1183Y mutations characteristic for the SARS-CoV-2 eta variant (B.1.429).
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Key Features
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- Recombinant trimeric S protein of the SARS-CoV-2 variant of concern B.1.1.7.
- Contains del 69-70, del 144, N501Y, A570D, D614G, P681H, T716I, S982A, and D1118H mutations characteristic for the SARS-CoV-2 alpha variant (B.1.1.7).
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Key Features
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- Recombinant trimeric S protein of the SARS-CoV-2 variant of concern P.1.
- Contains L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, H655Y, T1027I, and V1176F mutations characteristic for the SARS-CoV-2 gamma variant (P.1).
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Key Features
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- Recombinant trimeric S protein of the SARS-CoV-2 variant of concern B.1.617.
- Contains T19R, L452R, T478K, D614G, P681R and D950N mutations characteristic for the SARS-CoV-2 delta variant (B.1.617.2).
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Key Features
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- Recombinant trimeric S protein of the SARS-CoV-2 variant of interest B.1.621 mu.
- Contains T95I, Y144S, Y145N, R346K, E484K, N501Y, D614G, P681H and D950N mutations characteristic for the SARS-CoV-2 mu variant (B.1.621).
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Key Features
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- NLRP3 antibody for reliable recognition of mouse and human NLRP3/NALP3
- This un-conjugated monoclonal antibody has 60 PubMed citations
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Key Features
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- 95+ publication references, 1 independent validation
- Frequently used as CUT&RUN IgG negative control and CUT&Tag secondary antibody
- Used in CUT&RUN and CUT&Tag protocols, e.g. Henikoff et al. (2018) PMID 29651053, Kaya-Okur et al. (2019, 2020) PMID 31036827 and PMID 32913232
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Key Features
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- Recombinant trimeric S protein of the SARS-CoV-2 variant of concern B.1.1.529 omicron (21K).
- Contains A67V, H69del, V70del, T95I, G142del, V143del, Y144del, Y145D, N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y, N856K, Q954H, N969K, and L981F mutations characteristic for the SARS-CoV-2 omicron variant (B.1.1.529).
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Key Features
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- Recombinant S protein S1+S2 ECD (M1-P1213) of the SARS-CoV-2 variant of concern B.1.617.2 delta expressed in insect cells.
- Contains T19R, G142D, E156G, FR157-158 deletion, L452R, T478K, D614G, P681R, and D950N mutations characteristic for the SARS-CoV-2 delta variant (B.1.617.2).
- Intact furin cleavage site 680-SRRRAR|SV-687.
- C-terminal His-tag.
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Key Features
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- Recombinant S protein S1+S2 ECD (M1-P1213) of the SARS-CoV-2 variant of concern B.1.617.2 delta expressed in insect cells.
- Contains EFR157-158 deletion, T19R, G142D, E156G, FR157-158 deletion, L452R, T478K, D614G, P681R, and D950N mutations characteristic for the SARS-CoV-2 delta variant (B.1.617.2).
- Intact furin cleavage site 680-SRRRAR|SV-687.
- C-terminal His-tag.
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Key Features
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- Recombinant S protein S1+S2 ECD (M1-P1213) of the SARS-CoV-2 variant of concern P.1 gamma expressed in insect cells.
- Contains L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, and T1027I mutations characteristic for the SARS-CoV-2 gamma variant (P.1).
- Intact furin cleavage site 680-SPRRAR|SV-687.
- C-terminal His-tag.
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Key Features
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- Recombinant S protein S1+S2 ECD (M1-P1213) of the SARS-CoV-2 variant of concern P.1 gamma expressed in insect cells.
- Contains L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, and V1176F mutations characteristic for the SARS-CoV-2 gamma variant (P.1).
- Intact furin cleavage site 680-SPRRAR|SV-687.
- C-terminal His-tag.
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Key Features
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- Recombinant S protein S1+S2 (M1-P1213) of the SARS-CoV-2 variant of concern B.1.351 expressed in insect cells.
- Contains D80A, K417N, E484K, N501Y, D614G, and A701V mutations characteristic for the SARS-CoV-2 beta variant (B.1.351).
- Intact furin cleavage site 680-SPRRAR|SV-687.
- C-terminal His-tag.
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Key Features
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- Recombinant S protein S1+S2 ECD (M1-P1213) of the SARS-CoV-2 variant of concern B.1.351 expressed in insect cells.
- Contains L18F, D80A, D215G, LAL242-244 deletion, R246I, K417N, E484K, N501Y, D614G, and A701V mutations characteristic for the SARS-CoV-2 beta variant (B.1.351).
- Intact furin cleavage site 680-SPRRAR|SV-687.
- C-terminal His-tag.
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Key Features
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- Recombinant S protein S1+S2 (M1-P1213) of the SARS-CoV-2 variant of concern B.1.351 expressed in insect cells.
- Contains L18F, D80A, D215G, LAL242-244 deletion, R246I, K417N, E484K, N501Y, D614G, and A701V mutations characteristic for the SARS-CoV-2 beta variant (B.1.351).
- Intact furin cleavage site 680-SPRRAR|SV-687.
- C-terminal His-tag.
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Key Features
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- Recombinant S protein S1+S2 ECD (M1-P1213) of the SARS-CoV-2 variant of concern B.1.1.7 expressed in insect cells.
- Contains HV69-70 deletion, Y145 deletion, N501Y, A570D, D614G, P681H, T716I, S982A, and D1118H characteristic for the SARS-CoV-2 alpha variant (B.1.1.7).
- Intact furin cleavage site 680-SHRRAR|SV-687.
- C-terminal His-tag.
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Key Features
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- Recombinant S protein S1+S2 ECD (M1-P1213) of the SARS-CoV-2 variant of concern B.1.1.7 expressed in insect cells.
- Contains HV69-70 deletion, Y144 deletion, N501Y, A570D, D614G, P681H, T716I, S982A, and D1118H characteristic for the SARS-CoV-2 alpha variant (B.1.1.7).
- Intact furin cleavage site 680-SHRRAR|SV-687.
- C-terminal His-tag.
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Key Features
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- Recombinant SARS-CoV-2 wt S protein S1+S2 (M1-P1213) expressed in insect cells.
- Contains D614G mutation.
- Intact furin cleavage site 680-SPRRAR|SV-687.
- C-terminal His-tag.
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Key Features
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- High-affinity single-domain antibody
- Recognizes mRFP, mCherry, mScarlet-i, tdTomato, dsRed etc.
- Bead size 50-150 μm
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Key Features
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- Monoclonal murine neutralizing antibody (nAb) against SARS-CoV-2 S protein.
- Binds SARS-CoV-2 S proteins of various lineages, including B.1.617.2 (delta) and B.1.1.529 (omicron).
- Ideal for SARS-CoV-2 S-protein ELISAs and neutralization assays.
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Key Features
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- Monoclonal murine neutralizing antibody (nAb) against SARS-CoV-2 S protein.
- Binds SARS-CoV-2 S proteins of various lineages, including B.1.617.2 (delta). Does NOT bind the S protein of SARS-CoV-2 B.1.1.529 (omicron).
- Ideal for SARS-CoV-2 S-protein ELISAs and neutralization assays.
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Key Features
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- Rabbit beta-Catenin antibody reliably detects beta-Catenin targets in Wnt-signaling using CUT&RUN, ICC, IF, WB, ELISA.
- Well suited to image adherens junctions in IHC/IF.
- Highly specific beta-Catenin detection in WB.
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Key Features
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- Rabbit anti-CRBN Antibody reliably detects CRBN in human species.
- Polyclonal, unconjugated antibody for ELISA, WB, IHC.
- Highly specific CRBN detection. See validation images.
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Key Features
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- Mouse IL-1 beta ELISA kit for quantitative detection of IL-1 beta.
- Reliable product with validated components.
- ELISA kit cited in more than 40 PubMed References.
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Key Features
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- Magnetic agarose Concanavalin A beads for affinity chromatography using a magnetic separator.
- Easy bench-top purification of viruses, virus-like particles (VLP), and cultured cells via reversible binding of glycoproteins, glycolipids, or polysaccharides with terminal mannose or glucose to concanavalin A.
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Key Features
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- Polyclonal, unconjugated GFP antibody for reliable detection of GFP and its variants.
- Validated for Fluorescence Microscopy, ELISA, Western Blotting
- High Quality GFP antibody, cited in more than 177 PubMed References.
- Available in 10 µl and 100 µl quantities.
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Key Features
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- Reliable Taurine Assay Kit for measurement of free taurine in biological samples.
- Sample taurine concentrations are determined by comparison with a known taurine standard.
- Quick and easy to use.
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Key Features
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- Impurity detection in the manufacturing of biological drugs.
- The AccuSignal™ Nuclease ELISA Kit is designed for sensitive and reliable quantification of nucleases in therapeutic products, including DENARASE®, Benzonase®, and Turbonuclease.
- Offers a broad quantification spectrum, maintaining outstanding dilution linearity, instilling trust in the accuracy of results over a wide array of nuclease concentrations.
- Delivers consistent and repeatable outcomes, characterized by minimal intra- and inter-assay variability.
- Tailored antibody specificity allows for application across diverse materials, accommodating various nuclease products from multiple suppliers.
- Components: 96-well strip plate, plate sealer, nuclease standard, biotinylated detection antibody, Streptavidin-HRO (100X), buffers.
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Key Features
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- High-affinity anti-GFP single-domain antibody (sdAb) covalently bound to 4 % cross-linked agarose magnetic beads with 50-150 µm diameter.
- The Alpaca sdAb clone 1H1 is specific for GFP and many GFP derivatives. Capacity > 3 μg GFP per μl of packed beads.
- Compatible with physiological buffers, common Lysis and Wash Buffers, and high stringency buffers.
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Key Features
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- High-quality FcRn antibody (Clone ADM31) for the detection of FcRn.
- Highly specific for human FcRn, blocks the binding of human serum albumin.
- PBS-only, preservative free
- 15 PubMed references available for this FcRn antibody.
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Key Features
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- High-quality FcRn antibody (Clone DVN24) for the detection of FcRn.
- Highly specific for human FcRn, blocks the binding of IgG.
- PBS-only, preservative free
- 2 PubMed references available for this FcRn antibody.
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Key Features
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- High-affinity anti-GFP single-domain antibody (sdAb) covalently bound to 4 % cross-linked agarose beads with 50-150 µm diameter.
- The Alpaca sdAb clone 1H1 is specific for GFP and many GFP derivatives. Capacity > 3 μg GFP per μl of packed beads.
- Compatible with physiological buffers, common Lysis and Wash Buffers, and high stringency buffers.
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Key Features
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- TrueBlot®: Anti-Rabbit IgG HRP, Mouse Monoclonal eB182
- Manufactured by Rockland Immunochemicals, Inc.
- Rockland Product ID: 18-8816-33
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Key Features
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- TrueBlot®: Anti-Rabbit IgG HRP, Mouse Monoclonal eB182
- Manufactured by Rockland Immunochemicals, Inc.
- Rockland Product ID: 18-8816-31
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Key Features
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- Anti-ATM Protein Kinase pS1981 (MOUSE) Monoclonal Antibody
- Manufactured by Rockland Immunochemicals, Inc.
- Rockland Product ID: 200-301-400
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Key Features
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- High-quality FcRn antibody (Clone ADM31) for the detection of FcRn.
- Highly specific for human FcRn, blocks the binding of human serum albumin.
- contains Glycerol for better storage.
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Key Features
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- High-quality FcRn antibody (Clone DVN24) for the detection of FcRn.
- Highly specific for human FcRn, blocks the binding of IgG.
- Contains Glycerol for better storage.
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原理
- The HDL Purification Kit uses Dextran Sulfate to selectively precipitate HDL.
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产品特性
- The kit allows for the purification of HDL without the need for ultracentrifugation. The lipoprotein particles are highly purified through a series of precipitation and low speed centrifugation steps. Each kit provides sufficient reagents to perform up to 10 preps, and each preparation can purify up to 10 mL of serum or plasma samples with a yield of ~4500 μg of HDL per mL for human samples (expected yield will vary by species).
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组件
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- Dextran Solution : One 8 mL bottle
- Precipitation Solution A : One 30 mL amber bottle
- Tris Solution : One 50 mL bottle containing 20 mM Tris, pH 7.5
- Dextran Removal Solution : One 10 mL bottle
- 5X HDL Wash Solution : One 12 mL bottle
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试剂未包括
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- Serum or Plasma Samples
- PBS
- Microcentrifuge or Centrifuge
- 10 μL to 1000 μL adjustable single channel micropipettes with disposable tips
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Product Specific Information
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Our GFP Catcher is a product based on the GFP pull-down technique used to isolate GFP fusion proteins from cell lysates. It consists of special magnetic beads (product ABIN7272855) or agarose beads (product ABIN5311508) coated with an antibody against GFP. These beads allow efficient and selective binding to GFP or GFP-fused target proteins.
The application of GFP Catcher is similar to the GFP pull-down method. First, the protein of interest is expressed in the cells with it fused to GFP. Then, the cell lysate is prepared to extract the target proteins. The GFP catcher beads are then added to the cell lysate and bound to these proteins by specifically binding to the GFP or GFP fusion protein.
After the beads with the bound target proteins are isolated, thorough purification is performed to remove components that are not specifically bound. Finally, the target proteins can be separated from the beads and used for further analysis such as immunoblotting or mass spectrometry.
GFP Catcher offers the advantage of quick and easy isolation of GFP fusion proteins. It is a useful tool for protein analysis and allows efficient identification of proteins interacting with GFP fusion protein. GFP Catcher is widely used in cell biology research to study protein-protein interactions and deepen the understanding of cellular processes.
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应用备注
- Optimal working dilution should be determined by the investigator.
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试剂准备
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- HDL Resuspension Buffer: Dilute Dextran Solution 1:100 and Precipitation Solution A 1:10 in Tris Solution. For example, add 50 μL of Dextran Solution and 0.5 mL of Precipitation Solution A to 4.45 mL of Tris Solution. Stir to homogeneity. Prepare only enough for immediate use and do not store unused buffer. 3
- 1X HDL Wash Solution: Dilute the 5X HDL Wash Solution to 1X with deionized water. Stir to homogeneity. Store unused solution at 4 °C. Purification Protocol Note: The purification protocol below is written for a 10 mL sample size. For smaller sample volumes, scale down each step proportionally. I. Dextran Precipitation 1. To 10 mL of serum or plasma on ice, add 50 μL of Dextran Solution and 500 μL of Precipitation Solution A. Incubate 5 minutes on ice. 2. Spin at 6000 x g 10 minutes at 4 °C. 3. Transfer the supernatant to a new tube for use in section II below. Discard the pellet. II. HDL Purification 1. To 10 mL of supernatant from section I above, add 600 μL of Dextran Solution and 1.5 mL of Precipitation Solution A. Incubate for 2 hours at room temperature. 2. Spin 18,000-20,000 x g for 30 minutes at 4 °C. 3. Discard supernatant and resuspend pellet in 5 mL of HDL Resuspension Buffer (see Preparation of Reagents Section). Mix thoroughly by pipetting up and down. 4. Spin 6000 x g for 10 minutes at 4 °C. 5. Discard supernatant and resuspend pellet in 6 mL of 1X HDL Wash Solution (see Preparation of Reagents Section). 6. Shake for 30 minutes at 4 °C. Shaking speed should be sufficient to dissolve pellet, but not so vigorous that bubbles form. 7. Spin 6000 x g for 10 minutes at 4 °C. 8. Transfer the supernatant to a new tube and add 900 μL of Dextran Removal Solution. Mix thoroughly by pipetting up and down. 9. Incubate for 1 hour at 4 °C. 10. Spin 6000 x g for 10 minutes at 4 °C. 11. Transfer the supernatant (containing purified HDL) to a new tube. 12. Dialyze the purified HDL in PBS and determine the protein concentration. 4
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Upon receipt store Dextran Removal Solution at room temperature. Store all other components at 4°C.
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: "Refined purification strategy for reliable proteomic profiling of HDL2/3: Impact on proteomic complexity." in: Scientific reports, Vol. 6, pp. 38533, (2016) (PubMed).
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: "Refined purification strategy for reliable proteomic profiling of HDL2/3: Impact on proteomic complexity." in: Scientific reports, Vol. 6, pp. 38533, (2016) (PubMed).
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背景
- Lipoproteins are submicroscopic particles composed of lipid and protein held together by noncovalent forces. Their general structure is that of a putative spheroidal microemulsion formed from an outer layer of phospholipids, unesterified cholesterol, and proteins, with a core of neutral lipids, predominately cholesteryl esters and triacylglycerols (TAG). Very low density lipoprotein (VLDL), a spherical particle with a diameter of 30-100 nm, is the major plasma vehicle for TAG and is the precursor to Low density lipoprotein (LDL). Each VLDL contains one molecule of a hydrophobic protein known as apolipoprotein B-100 (Apo B), as well as multiple copies of apolipoprotein E and apolipoprotein C. High Density Lipoprotein (HDL) is also a spherical particle with diameter of about 10 nm (Figure 1). HDL contains the Apoliprotein AI and AII molecules. HDL and LDL cholesterol levels in the blood are important indicators of many disease states. High blood levels of LDLs are associated with health problems and cardiovascular disease. For this reason, LDL is often referred to as the "bad cholesterol." LDL particles that accumulate within arteries can form plaques over time, which can increase chances of a stroke, heart attack, or vascular disease. HDL particles are able to remove cholesterol from within arteries and transport it back to the liver for re-utilization or excretion, which is the main reason why the cholesterol carried within HDL particles is sometimes called "good cholesterol." Monitoring circulatory levels of different lipoproteins is critical to the diagnosis of lipid transport disorders such as atherosclerosis. Figure 1: Structure of HDL.
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