IL-7 ELISA 试剂盒
Quick Overview for IL-7 ELISA 试剂盒 (ABIN2112003)
抗原
See all IL-7 (IL7) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 1.5 pg/mL
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灵敏度
- < 1 pg/mL
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组件
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IL-7 MICROTITER PLATE
BIOTIN CONJUGATE
AVIDIN CONJUGATE
IL-7 STANDARD
CALIBRATOR DILUENT I
CALIBRATOR DILUENT II
WASH BUFFER (20X/96 wells, 30X/192 wells)
SUBSTRATE A
SUBSTRATE B
STOP SOLUTION
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Interleukin 7 (IL7) ELISA Kit
IL7 适用: Pig Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 7 (IL7) ELISA KitIL7 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 7 (IL7) ELISA KitIL7 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 7 (IL7) ELISA KitIL7 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 7 (IL7) ELISA KitSmall Sample IL7 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 7 (IL7) ELISA KitIL7 适用: 小鼠 AA 26-154 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Serum
Interleukin 7 (IL7) ELISA KitIL7 适用: 人 AA 26-177 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Serum
Interleukin 7 (IL7) ELISA KitIL7 适用: 人 Colorimetric Sandwich ELISA 32-2000 pg/mL Cell Lysate, Plasma, Serum
Interleukin 7 (IL7) ELISA KitIL7 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Serum
Interleukin 7 (IL7) ELISA KitIL7 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Serum
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板类型
- Pre-coated
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实验流程
- This IL-7 ELISA is a ready-to-use 3.5-hour solid phase immunoassay readily capable of measuring IL-7 levels in serum, plasma, cell culture supernatant, and other biological fluids in the range of 0 to 2000pg/ml. This assay has shown no cross-reactivity with other cytokines tested, and is expected to be used effectively for further investigations into the relationship between IL-7 and the various conditions mentioned.This IL-7 enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to IL-7. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IL-7 and incubated. If present, IL-7 will bind and become immobilized by the antibody pre-coated on the wells and then become -sandwiched- by biotin conjugate. The microtiter plate wells are thoroughly washed to remove unbound IL- 7 and other components of the sample. In order to quantitatively determine the amount of IL-7 present in the sample, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Avidin is a tetramer containing four identical subunits, each having a high affinity-binding site for biotin. The wells are thoroughly washed to remove all unbound HRP-conjugated Avidin and a TMB (3,3'5,5' tetramethyl- benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL-7, biotin- conjugated antibody, and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- IL-7 (IL7) (Interleukin 7 (IL7))
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别名
- Interleukin-7
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途径
- JAK/STAT Signaling
抗原 See all IL-7 (IL7) ELISA试剂盒
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