IL-5 ELISA 试剂盒
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Quick Overview for IL-5 ELISA 试剂盒 (ABIN2112000)
抗原
See all IL-5 (IL5) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 3.12 pg/mL
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灵敏度
- < 0.8 pg/mL
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组件
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MOUSE IL-5 MICROTITER PLATE
BIOTIN CONJUGATE
AVIDIN CONJUGATE
MOUSE IL-5 STANDARD
CALIBRATOR DILUENT I
CALIBRATOR DILUENT II
WASH BUFFER (20X/96 wells, 30X/192 wells)
TMB SUBSTRATE
STOP SOLUTION
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Interleukin 5 (IL5) ELISA Kit
IL5 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 5 (IL5) ELISA KitIL5 适用: Pig Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 5 (IL5) ELISA KitIL5 适用: 山羊 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 5 (IL5) ELISA KitIL5 适用: 绵羊 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 5 (IL5) ELISA KitIL5 适用: 人 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 5 (IL5) ELISA KitIL5 适用: 大鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 5 (IL5) ELISA KitIL5 适用: 小鸡 Colorimetric Sandwich ELISA 3.12 pg/mL - 200 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 5 (IL5) ELISA KitIL5 适用: 犬 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 5 (IL5) ELISA KitIL5 适用: 兔 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 5 (IL5) ELISA KitIL5 适用: Cow Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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实验流程
- Mouse IL-5 is a heamotapoietic cytokine, mainly produced by T-helper 2 cells and mast cells. The cytokine is comprised of two identical 113 amino acid units linked by disulfide bond. The gene for mouse IL-5 is located at chromosome 11 in conjunction with genes for IL4 and other T-helper 2 cytokines such as IL-13, GM-CSF, IL-3. IL-5 regulates B cell proliferation, eosinophil maturation and activation. IL-5 receptor is a heterodimer with a cytokine specific alpha unit, and a signal transduction beta subunit that is identical to the beta subunit of receptors for IL-3 and GM-CSF. IL-5 receptor alpha (IL-5Ra) is expressed by eosinophils, B cells and basophils in member-bound form and soluble form. While the membrane bound form participates in the activation of eosinophils and B cells, the soluble form of IL-5Ra has an antagonist effect to IL-5. Increased IL-5 expression causes chronic IgE response and hypereosinophilic syndromes. Elevated IL-5 level is found in allergic diseases such as allergic rhinitis, asthma and allergic conjunctivitis. This ELISA kit provides a tool for studying IL-5 expression and regulation in animal model. This mouse IL-5 enzyme-linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for mouse IL-5. When standards or samples are added to the appropriate microtiter plate wells, mouse IL-5 in the standards or samples will be immobilized by the precoated antibody during incubation. Then, a biotin-conjugated antibody preparation specific for mouse IL-5 is added to each well and incubated.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- IL-5 (IL5) (Interleukin 5 (IL5))
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别名
- Interleukin-5
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途径
- JAK/STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour
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