Interleukin 17a ELISA 试剂盒
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Quick Overview for Interleukin 17a ELISA 试剂盒 (ABIN2111994)
抗原
See all Interleukin 17a (IL17A) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 15.6 pg/mL
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灵敏度
- < 2 pg/mL
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组件
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ANTI-MOUSE IL-17A MICROTITER PLATE
BIOTIN CONJUGATE
AVIDIN CONJUGATE
MOUSE IL-17 STANDARD
CALIBRATOR DILUENT I
CALIBRATOR DILUENT II
WASH BUFFER (20X/96 wells, 30X/192 wells)
SUBSTRATE A
SUBSTRATE B
STOP SOLUTION
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Interleukin 17A (IL17A) ELISA Kit
IL17A 适用: 大鼠 Colorimetric Sandwich ELISA 93.75 pg/mL - 6000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 17A (IL17A) ELISA KitIL17A 适用: 人 Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Cell Culture Supernatant, Serum, Tissue Homogenate, Urine
Interleukin 17A (IL17A) ELISA KitIL17A 适用: 小鼠 AA 22-158 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Interleukin 17A (IL17A) ELISA KitIL17A 适用: 人 AA 20-155 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Interleukin 17A (IL17A) ELISA KitIL17A 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 17A (IL17A) ELISA KitIL17A 适用: 小鼠 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 17A (IL17A) ELISA KitIL17A 适用: 小鼠 Colorimetric Sandwich ELISA 6-600 pg/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 17A (IL17A) ELISA KitIL17A 适用: 大鼠 Colorimetric Sandwich ELISA 3.13-200 pg/mL Cell Culture Supernatant, Plasma, Serum
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板类型
- Pre-coated
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实验流程
- This mouse IL-17A ELISA is a 3.5-hour solid phase immunoassay readily applicable to measure mouse IL-17A levels in cell culture supernatant and mouse serum in the range of 0 to 1000pg/ml. It showed no cross reactivity with other cytokines tested. This mouse IL-17A ELISA is expected to be effectively used for further investigations into the relationship between IL-17A and the various conditions mentioned. This mouse IL-17A enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to mouse IL-17A. Standards or samples are then added to the appropriate microtiter plate wells. A biotin-conjugated antibody preparation specific for mouse IL-17A was added and incubated. Mouse IL-17A, if present, will bind and become immobilized by the antibody pre-coated on the wells. The microtiter plate wells are thoroughly washed to remove unbound mouse IL-17A and other components in the sample. Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Avidin is a tetramer containing four identical subunits that each has a high affinity-binding site for biotin. The wells are thoroughly washed to remove all unbound HRP-conjugated Avidin, and aTMB(3,3'5, 5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain mouse IL-17A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- Interleukin 17a (IL17A) (Interleukin 17A (IL17A))
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别名
- Interleukin-17A
抗原 See all Interleukin 17a (IL17A) ELISA试剂盒
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