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IL-13 ELISA 试剂盒

IL13 适用: 人 Colorimetric Sandwich ELISA 15.6-1000 pg/mL
产品编号 ABIN2111993
发货至: 中国
  • 抗原 See all IL-13 (IL13) ELISA试剂盒
    IL-13 (IL13) (Interleukin 13 (IL13))
    适用
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    15.6-1000 pg/mL
    最低检测浓度
    15.6 pg/mL
    应用范围
    ELISA
    品牌
    BioAssay™
    Analytical Method
    Quantitative
    灵敏度
    < 2 pg/mL
    组件
    L-13 MICROTITER PLATE
    BIOTIN CONJUGATE
    AVIDIN CONJUGATE
    IL-13 STANDARD
    CALIBRATOR DILUENT I
    CALIBRATOR DILUENT II
    WASH BUFFER (20X/96 wells, 30X/192 wells)
    SUBSTRATE A
    SUBSTRATE B
    STOP SOLUTION
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    Discover our top product IL13 ELISA Kit
  • 板类型
    Pre-coated
    实验流程
    This assay applies the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific to IL-13 has been pre-coated onto a microplate. Standards and samples are then added to the appropriate plate wells with a biotin-conjugated antibody preparation specific for IL-13 and incubated. IL-13 if present, will bind and become immobilized by the antibody pre-coated on the wells and then be "sandwiched" by biotin conjugate. Avidin is a tetramer containing four identical subunits that each has a high affinity-binding site for biotin. After washing away any unbound substances, avidin-Horseradish Peroxidase (HRP) will be added to each well and incubated. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and only those wells that contain IL-13, biotin conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The color development is stopped and the intensity of the color is measured.
    限制
    仅限研究用
  • 抗原 See all IL-13 (IL13) ELISA试剂盒
    IL-13 (IL13) (Interleukin 13 (IL13))
    别名
    Interleukin-13 (IL13 产品)
    别名
    IL13 ELISA Kit, IL-13 ELISA Kit, P600 ELISA Kit, Il-13 ELISA Kit, L13 ELISA Kit, interleukin 13 ELISA Kit, IL13 ELISA Kit, Il13 ELISA Kit
    背景
    IL-13 is also associated with many features of the allergic airway diseases, including mucus hypersecretion, goblet metaplasia, chemokine induction and recruitment of effector cells. All of them contribute to airway obstruction. Polymorphisms in IL-13 gene have been found to link to increased eosinophil count, serum total IgE and high risk of asthma.
    途径
    JAK/STAT Signaling, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport
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