IL-13 ELISA 试剂盒
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- 抗原 See all IL-13 (IL13) ELISA试剂盒
- IL-13 (IL13) (Interleukin 13 (IL13))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 15.6-1000 pg/mL
- 最低检测浓度
- 15.6 pg/mL
- 应用范围
- ELISA
- 品牌
- BioAssay™
- Analytical Method
- Quantitative
- 灵敏度
- < 2 pg/mL
- 组件
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L-13 MICROTITER PLATE
BIOTIN CONJUGATE
AVIDIN CONJUGATE
IL-13 STANDARD
CALIBRATOR DILUENT I
CALIBRATOR DILUENT II
WASH BUFFER (20X/96 wells, 30X/192 wells)
SUBSTRATE A
SUBSTRATE B
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- 板类型
- Pre-coated
- 实验流程
- This assay applies the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific to IL-13 has been pre-coated onto a microplate. Standards and samples are then added to the appropriate plate wells with a biotin-conjugated antibody preparation specific for IL-13 and incubated. IL-13 if present, will bind and become immobilized by the antibody pre-coated on the wells and then be "sandwiched" by biotin conjugate. Avidin is a tetramer containing four identical subunits that each has a high affinity-binding site for biotin. After washing away any unbound substances, avidin-Horseradish Peroxidase (HRP) will be added to each well and incubated. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and only those wells that contain IL-13, biotin conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The color development is stopped and the intensity of the color is measured.
- 限制
- 仅限研究用
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- 抗原 See all IL-13 (IL13) ELISA试剂盒
- IL-13 (IL13) (Interleukin 13 (IL13))
- 别名
- Interleukin-13 (IL13 产品)
- 别名
- IL13 ELISA Kit, IL-13 ELISA Kit, P600 ELISA Kit, Il-13 ELISA Kit, L13 ELISA Kit, interleukin 13 ELISA Kit, IL13 ELISA Kit, Il13 ELISA Kit
- 背景
- IL-13 is also associated with many features of the allergic airway diseases, including mucus hypersecretion, goblet metaplasia, chemokine induction and recruitment of effector cells. All of them contribute to airway obstruction. Polymorphisms in IL-13 gene have been found to link to increased eosinophil count, serum total IgE and high risk of asthma.
- 途径
- JAK/STAT Signaling, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport
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