GM-CSF ELISA 试剂盒
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Quick Overview for GM-CSF ELISA 试剂盒 (ABIN2111989)
抗原
See all GM-CSF (CSF2) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 50 pg/mL
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Analytical Method
- Quantitative
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灵敏度
- < 17 pg/mL
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组件
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MOUSE GM-CSF MICROTITER PLATE
BIOTIN CONJUGATE
AVIDIN CONJUGATE
MOUSE GM-CSF STANDARD
CALIBRATOR DILUENT I
CALIBRATOR DILUENT II
WASH BUFFER (20X/96 wells, 30X/192 wells)
SUBSTRATE A
SUBSTRATE B
STOP SOLUTION
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Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) ELISA Kit
CSF2 适用: 小鼠 Colorimetric Sandwich ELISA 1.37 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) ELISA KitCSF2 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) ELISA KitCSF2 适用: 大鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) ELISA KitCSF2 适用: 兔 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) ELISA KitCSF2 适用: Cow Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) ELISA KitCSF2 适用: 犬 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) ELISA KitCSF2 适用: 大鼠 AA 1-127 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) ELISA KitCSF2 适用: 人 AA 18-144 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) ELISA KitCSF2 适用: 小鼠 AA 18-141 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
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板类型
- Pre-coated
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实验流程
- This ELISA kit provides a tool for studying GM-CSF expression and regulation in animal model.This mouse GM-CSF enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for mouse GM-CSF. Standards or samples are then added to the appropriate microtiter plate wells and incubated. Mouse GM-CSF, if present, will bind and become immobilized by the antibody pre-coated on the wells. The microtiter plate wells are thoroughly washed to remove unbound mouse GM- CSF and other components of the sample. In order to quantitatively determine the amount of mouse GM-CSF present in the sample, a standardized preparation of horseradish peroxidase HRP-conjugated monoclonal antibody specific for mouse GM-CSF is added to each well to -sandwich- the mouse GM-CSF immobilized during the first incubation. The microtiter plate then undergoes a second incubation. The wells are thoroughly washed to remove all unbound HRP-conjugated antibodies and a TMB (3,3-5,5- tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain mouse GM-CSF and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 ± 2nm.
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限制
- 仅限研究用
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- GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))
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别名
- Granulocyte Macrophage Colony Stimulating Factor
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途径
- JAK/STAT Signaling, Cellular Response to Molecule of Bacterial Origin
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