Granulin ELISA 试剂盒
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Quick Overview for Granulin ELISA 试剂盒 (ABIN2111984)
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检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.06 ng/mL
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Analytical Method
- Quantitative
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灵敏度
- 32 pg/mL
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组件
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Plate coated with human progranulin antibody, 1x96wells
Wash Buffer 10X, 1x50 mL
Diluent 5X, 1x50 mL
Detection Antibody, 1x10 mL
Detector 100X (HRP Labeled Streptavidin), 1x150 µL
Human progranulin Standard, lyophilized, 1x8 ng
Human progranulin QC sample, lyophilized, 1x vial
TMB Substrate Solution, 1x10 mL
Stop Solution, 1x10 mL
Plate sealers, 3x plastic film
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Granulin (GRN) ELISA Kit
GRN 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Granulin (GRN) ELISA KitGRN 适用: 小鼠 Colorimetric Competition ELISA 0.469 ng/mL - 30 ng/mL Plasma, Serum, Tissue Homogenate
Granulin (GRN) ELISA KitGRN 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Granulin (GRN) ELISA KitGRN 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Granulin (GRN) ELISA KitGRN 适用: 人 AA 18-593 Colorimetric Sandwich ELISA 1.56-100 ng/mL Cell Culture Supernatant, Milk, Plasma (EDTA), Plasma (heparin), Saliva, Serum, Urine
Granulin (GRN) ELISA KitGRN 适用: 小鼠 AA 18-589 Colorimetric Sandwich ELISA 0.78-50 ng/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Granulin (GRN) ELISA KitGRN 适用: 人 Colorimetric Sandwich ELISA 63-4000 pg/mL Cell Lysate, Plasma, Serum
Granulin (GRN) ELISA KitGRN 适用: 小鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Plasma, Serum
Granulin (GRN) ELISA KitGRN 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Breast Milk, Cell Culture Supernatant, Plasma (EDTA - heparin), Saliva, Serum, Urine
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板类型
- Pre-coated
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实验流程
- This BioAssay-is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of human progranulin in biological fluids. A polyclonal antibody specific for progranulin has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, progranulin is recognized by the addition of a biotinylated polyclonal antibody specific for progranulin (Detection Antibody). After removal of excess biotinylated antibody, HRP labeled streptavidin (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3-,5,5--tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450nm after acidification and is directly proportional to the concentration of progranulin in the samples.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- Granulin (GRN)
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别名
- Progranulin
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物质类
- Viral Protein
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背景
- Progranulin (PGRN) is a widely expressed pluripotent growth factor which plays a role in processes such as development, wound repair and inflammation by activating signaling cascades that control cell cycle progression and cell motility. Its function in the central nervous system is of interest, as mutations in the PGRN gene were found in cases of frontotemporal degeneration (FTLD). In addition, PGRN has also been linked to tumorigenesis. New cutting-edge publications have shown that progranulin might be a biomarker not only for FTLD, but also for other types of Alzheimer-s disease (AD). This new data even supposes the potential of progranulin to be a biomarker for MCI (Mild Cognitive Impairment), which would be a hallmark in AD research. Additionally, PGRN is described as a new ligand of TNF receptors and a potential therapeutic against inflammatory disease like arthritis.
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