WWP2 ELISA 试剂盒
人 WWP2 ELISA Kit, Colorimetric is an assay for quantification of 人 WWP2.
产品编号 ABIN2044855
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
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Beijing Economic Technological Development Area
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Quick Overview for WWP2 ELISA 试剂盒 (ABIN2044855)
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WWP2
(WW Domain Containing E3 Ubiquitin Protein Ligase 2 (WWP2))
适用
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
0.78-50 ng/mL
应用范围
ELISA
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最低检测浓度
- 0.78 ng/mL
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灵敏度
- < 0.27 ng/mL
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WW Domain Containing E3 Ubiquitin Protein Ligase 2 (WWP2) ELISA Kit
WWP2 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Lysate, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- WW Domain Containing E3 Ubiquitin Protein Ligase 2 (WWP2) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to WW Domain Containing E3 Ubiquitin Protein Ligase 2 (WWP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to WW Domain Containing E3 Ubiquitin Protein Ligase 2 (WWP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain WW Domain Containing E3 Ubiquitin Protein Ligase 2 (WWP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of WW Domain Containing E3 Ubiquitin Protein Ligase 2 (WWP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- WWP2 (WW Domain Containing E3 Ubiquitin Protein Ligase 2 (WWP2))
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途径
- Negative Regulation of Transporter Activity
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