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Vitamin D-Binding Protein ELISA 试剂盒

This 大鼠 Vitamin D-Binding Protein ELISA Kit is a Colorimetric ELISA Kit designed to quantify 大鼠 Vitamin D-Binding Protein.

产品编号 ABIN2044799

发货至: 中国

Quick Overview for Vitamin D-Binding Protein ELISA 试剂盒 (ABIN2044799)

抗原

See all Vitamin D-Binding Protein (GC) ELISA试剂盒

Vitamin D-Binding Protein (GC)

适用

15
12
8
4
2
2
2
2
2
2
1
1
1

大鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

12.5-800 ng/mL

应用范围

ELISA

Discover our top product GC ELISA Kit

最低检测浓度

12.5 ng/mL

灵敏度

< 5.57 ng/mL
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板类型

Pre-coated

实验流程

Vitamin D Binding Protein (DBP) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vitamin D Binding Protein (DBP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vitamin D Binding Protein (DBP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vitamin D Binding Protein (DBP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Vitamin D Binding Protein (DBP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

限制

仅限研究用
储存条件

4 °C/-20 °C
抗原 See all Vitamin D-Binding Protein (GC) ELISA试剂盒

Vitamin D-Binding Protein (GC)

别名

Vitamin D Binding Protein (DBP)

途径

Metabolism of Steroid Hormones and Vitamin D, Monocarboxylic Acid Catabolic Process

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