UACA ELISA 试剂盒
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Quick Overview for UACA ELISA 试剂盒 (ABIN2044767)
抗原
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检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 0.31 ng/mL
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灵敏度
- < 0.312-20 ng/mL
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Uveal Autoantigen With Coiled Coil Domains And Ankyrin Repeats (UACA) ELISA Kit
UACA 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Tissue Homogenate
Uveal Autoantigen With Coiled Coil Domains And Ankyrin Repeats (UACA) ELISA KitUACA 适用: 人 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Uveal Autoantigen With Coiled Coil Domains And Ankyrin Repeats (UACA) ELISA KitUACA 适用: 小鼠 Colorimetric Competition ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- Uveal Autoantigen With Coiled Coil Domains And Ankyrin Repeats (UACA) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Uveal Autoantigen With Coiled Coil Domains And Ankyrin Repeats (UACA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Uveal Autoantigen With Coiled Coil Domains And Ankyrin Repeats (UACA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Uveal Autoantigen With Coiled Coil Domains And Ankyrin Repeats (UACA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Uveal Autoantigen With Coiled Coil Domains And Ankyrin Repeats (UACA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- UACA (Uveal Autoantigen With Coiled Coil Domains And Ankyrin Repeats (UACA))
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途径
- Positive Regulation of Endopeptidase Activity
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