ISG15 ELISA 试剂盒
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Quick Overview for ISG15 ELISA 试剂盒 (ABIN2044741)
抗原
See all ISG15 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 31.25 pg/mL
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灵敏度
- < 13.5 pg/mL
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ISG15 Ubiquitin-Like Modifier (ISG15) ELISA Kit
ISG15 适用: 小鼠 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
ISG15 Ubiquitin-Like Modifier (ISG15) ELISA KitISG15 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Serum, Tissue Homogenate
ISG15 Ubiquitin-Like Modifier (ISG15) ELISA KitISG15 适用: Cow Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
ISG15 Ubiquitin-Like Modifier (ISG15) ELISA KitISG15 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
ISG15 Ubiquitin-Like Modifier (ISG15) ELISA KitISG15 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
ISG15 Ubiquitin-Like Modifier (ISG15) ELISA KitISG15 适用: Cow Colorimetric Sandwich ELISA 0.312-20 ng/mL Plasma, Serum, Tissue Homogenate
ISG15 Ubiquitin-Like Modifier (ISG15) ELISA KitISG15 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- Ubiquitin Cross Reactive Protein (UCRP) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ubiquitin Cross Reactive Protein (UCRP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ubiquitin Cross Reactive Protein (UCRP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ubiquitin Cross Reactive Protein (UCRP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Ubiquitin Cross Reactive Protein (UCRP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- ISG15 (ISG15 Ubiquitin-Like Modifier (ISG15))
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别名
- Ubiquitin Cross Reactive Protein (UCRP)
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