UQCR11 ELISA 试剂盒
This 人 UQCR11 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 UQCR11.
Quick Overview for UQCR11 ELISA 试剂盒 (ABIN2044735)
抗原
See all UQCR11 products
UQCR11
(Ubiquinol-Cytochrome C Reductase, Complex III Subunit XI (UQCR11))
适用
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
0.78-50 ng/mL
应用范围
ELISA
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最低检测浓度
- 0.78 ng/mL
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灵敏度
- < 0.29 ng/mL
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板类型
- Pre-coated
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实验流程
- Ubiquinol Cytochrome C Reductase (UQCR) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ubiquinol Cytochrome C Reductase (UQCR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ubiquinol Cytochrome C Reductase (UQCR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ubiquinol Cytochrome C Reductase (UQCR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Ubiquinol Cytochrome C Reductase (UQCR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- UQCR11 (Ubiquinol-Cytochrome C Reductase, Complex III Subunit XI (UQCR11))
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别名
- Ubiquinol Cytochrome C Reductase (UQCR)
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途径
- Proton Transport
抗原 See all UQCR11 products
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