SPTLC2 ELISA 试剂盒
This 小鼠 SPTLC2 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 小鼠 SPTLC2.
Quick Overview for SPTLC2 ELISA 试剂盒 (ABIN2044527)
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SPTLC2
(serine Palmitoyltransferase, Long Chain Base Subunit 2 (SPTLC2))
适用
小鼠
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
0.15-10 ng/mL
应用范围
ELISA
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最低检测浓度
- 0.15 ng/mL
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灵敏度
- < 0.055 ng/mL
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serine Palmitoyltransferase, Long Chain Base Subunit 2 (SPTLC2) ELISA Kit
SPTLC2 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- Serine Palmitoyltransferase, Long Chain Base Subunit 2 (SPTLC2) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serine Palmitoyltransferase, Long Chain Base Subunit 2 (SPTLC2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Serine Palmitoyltransferase, Long Chain Base Subunit 2 (SPTLC2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Serine Palmitoyltransferase, Long Chain Base Subunit 2 (SPTLC2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Serine Palmitoyltransferase, Long Chain Base Subunit 2 (SPTLC2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- SPTLC2 (serine Palmitoyltransferase, Long Chain Base Subunit 2 (SPTLC2))
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