SEMG2 ELISA 试剂盒
Quick Overview for SEMG2 ELISA 试剂盒 (ABIN2044525)
抗原
See all SEMG2 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 0.31 ng/mL
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灵敏度
- < 0.114 ng/mL
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Semenogelin II (SEMG2) ELISA Kit
SEMG2 适用: 人 Colorimetric Sandwich ELISA 0.313 μg/mL - 20 μg/mL Plasma, Serum, Tissue Homogenate
Semenogelin II (SEMG2) ELISA KitSEMG2 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Seminal Plasma, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- Semenogelin II (SEMG2) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Semenogelin II (SEMG2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Semenogelin II (SEMG2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Semenogelin II (SEMG2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Semenogelin II (SEMG2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- SEMG2 (Semenogelin II (SEMG2))
抗原 See all SEMG2 ELISA试剂盒
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