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SKP2 ELISA 试剂盒

This 人 SKP2 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 SKP2.
产品编号 ABIN2044485
发货至: 中国

Quick Overview for SKP2 ELISA 试剂盒 (ABIN2044485)

抗原

See all SKP2 ELISA试剂盒
SKP2 (S-Phase Kinase-Associated Protein 2 (SKP2))

适用

  • 4
  • 1

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

0.15-10 ng/mL

应用范围

ELISA
  • 最低检测浓度

    0.15 ng/mL

    灵敏度

    < 0.061 ng/mL
  • 板类型

    Pre-coated

    实验流程

    S-Phase Kinase Associated Protein 2 (SKP2) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to S-Phase Kinase Associated Protein 2 (SKP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to S-Phase Kinase Associated Protein 2 (SKP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain S-Phase Kinase Associated Protein 2 (SKP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of S-Phase Kinase Associated Protein 2 (SKP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

    限制

    仅限研究用
  • 储存条件

    4 °C/-20 °C
  • 抗原 See all SKP2 ELISA试剂盒

    SKP2 (S-Phase Kinase-Associated Protein 2 (SKP2))

    别名

    S-Phase Kinase Associated Protein 2 (SKP2)

    途径

    Mitotic G1-G1/S Phases
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