RNASE1 ELISA 试剂盒
Quick Overview for RNASE1 ELISA 试剂盒 (ABIN2044443)
抗原
See all RNASE1 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 78.12 pg/mL
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灵敏度
- < 35 pg/mL
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Ribonuclease, RNase A Family, 1 (Pancreatic) (RNASE1) ELISA Kit
RNASE1 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Ribonuclease, RNase A Family, 1 (Pancreatic) (RNASE1) ELISA KitRNASE1 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum
Ribonuclease, RNase A Family, 1 (Pancreatic) (RNASE1) ELISA KitRNASE1 适用: Cow Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum, Tissue Homogenate
Ribonuclease, RNase A Family, 1 (Pancreatic) (RNASE1) ELISA KitRNASE1 适用: 大鼠 Colorimetric Sandwich ELISA 1.563-100 ng/mL Plasma, Serum
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板类型
- Pre-coated
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实验流程
- Ribonuclease A (RNASE1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ribonuclease A (RNASE1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ribonuclease A (RNASE1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ribonuclease A (RNASE1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Ribonuclease A (RNASE1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- RNASE1 (Ribonuclease, RNase A Family, 1 (Pancreatic) (RNASE1))
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别名
- Ribonuclease A (RNASE1)
抗原 See all RNASE1 ELISA试剂盒
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