RHD ELISA 试剂盒
Quick Overview for RHD ELISA 试剂盒 (ABIN2044433)
抗原
See all RHD ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 0.15 ng/mL
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灵敏度
- < 0.058 ng/mL
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Rh Blood Group, D Antigen (RHD) ELISA Kit
RHD 适用: 人 Colorimetric Competition ELISA 2.47 ng/mL - 200 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Rh Blood Group, D Antigen (RHD) ELISA KitRHD 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- Rh Blood Group, D Antigen (RHD) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rh Blood Group, D Antigen (RHD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Rh Blood Group, D Antigen (RHD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rh Blood Group, D Antigen (RHD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Rh Blood Group, D Antigen (RHD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- RHD (Rh Blood Group, D Antigen (RHD))
抗原 See all RHD ELISA试剂盒
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