RORC ELISA 试剂盒
Quick Overview for RORC ELISA 试剂盒 (ABIN2044383)
抗原
See all RORC ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 0.15 ng/mL
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灵敏度
- < 0.054 ng/mL
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RAR-Related Orphan Receptor C (RORC) ELISA Kit
RORC 适用: 小鼠 Colorimetric Sandwich ELISA 46.875 pg/mL - 3000 pg/mL Plasma, Serum, Tissue Homogenate
RAR-Related Orphan Receptor C (RORC) ELISA KitRORC 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
RAR-Related Orphan Receptor C (RORC) ELISA KitRORC 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
RAR-Related Orphan Receptor C (RORC) ELISA KitRORC 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
RAR-Related Orphan Receptor C (RORC) ELISA KitRORC 适用: 小鼠 Colorimetric Sandwich ELISA 46.9 pg/mL - 3000 pg/mL Plasma, Serum, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- RAR Related Orphan Receptor Gamma (RORg) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to RAR Related Orphan Receptor Gamma (RORg). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to RAR Related Orphan Receptor Gamma (RORg). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RAR Related Orphan Receptor Gamma (RORg), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of RAR Related Orphan Receptor Gamma (RORg) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- RORC (RAR-Related Orphan Receptor C (RORC))
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别名
- RAR Related Orphan Receptor gamma (RORg)
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途径
- Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway
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