MPZ ELISA 试剂盒
Quick Overview for MPZ ELISA 试剂盒 (ABIN2044355)
抗原
See all MPZ ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 0.15 ng/mL
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品牌
- BioAssay™
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灵敏度
- < 0.052 ng/mL
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Myelin Protein Zero (MPZ) ELISA Kit
MPZ 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Myelin Protein Zero (MPZ) ELISA KitMPZ 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- Protein Zero, Myelin (MPZ) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Zero, Myelin (MPZ). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Zero, Myelin (MPZ). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Zero, Myelin (MPZ), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Protein Zero, Myelin (MPZ) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- MPZ (Myelin Protein Zero (MPZ))
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别名
- Protein Zero, Myelin (MPZ)
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