PIAS3 ELISA 试剂盒
This Colorimetric ELISA kit is designed for the quantitative measurement of 人 PIAS3.
产品编号 ABIN2044325
发货至:
中国
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Contact Info
Our Local Distributor
中国
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北京 101111
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Quick Overview for PIAS3 ELISA 试剂盒 (ABIN2044325)
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PIAS3
(Protein Inhibitor of Activated STAT, 3 (PIAS3))
适用
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
0.15-10 ng/mL
应用范围
ELISA
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最低检测浓度
- 0.15 ng/mL
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灵敏度
- < 0.064 ng/mL
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Protein Inhibitor of Activated STAT, 3 (PIAS3) ELISA Kit
PIAS3 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- Protein Inhibitor Of Activated STAT 3 (PIAS3) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Inhibitor Of Activated STAT 3 (PIAS3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Inhibitor Of Activated STAT 3 (PIAS3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Inhibitor Of Activated STAT 3 (PIAS3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Protein Inhibitor Of Activated STAT 3 (PIAS3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- PIAS3 (Protein Inhibitor of Activated STAT, 3 (PIAS3))
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别名
- Protein Inhibitor Of Activated STAT 3 (PIAS3)
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途径
- JAK/STAT Signaling
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