Prostacyclin Receptor ELISA 试剂盒
This Colorimetric ELISA kit is designed for the quantitative measurement of 人 Prostacyclin Receptor.
产品编号 ABIN2044301
发货至:
中国
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中国
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北京 101111
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Quick Overview for Prostacyclin Receptor ELISA 试剂盒 (ABIN2044301)
抗原
See all Prostacyclin Receptor (PTGIR) ELISA试剂盒
Prostacyclin Receptor (PTGIR)
适用
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
0.15-10 ng/mL
应用范围
ELISA
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最低检测浓度
- 0.15 ng/mL
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灵敏度
- < 0.071 ng/mL
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Prostacyclin Receptor (PTGIR) ELISA Kit
PTGIR 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- Prostacyclin Receptor (PTGIR) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prostacyclin Receptor (PTGIR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Prostacyclin Receptor (PTGIR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Prostacyclin Receptor (PTGIR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Prostacyclin Receptor (PTGIR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- Prostacyclin Receptor (PTGIR)
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途径
- cAMP Metabolic Process, Platelet-derived growth Factor Receptor Signaling, Thromboxane A2 Receptor Signaling
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