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Podoplanin ELISA 试剂盒

This Colorimetric ELISA kit is designed for the quantitative measurement of 小鼠 Podoplanin.
产品编号 ABIN2044247
发货至: 中国
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Quick Overview for Podoplanin ELISA 试剂盒 (ABIN2044247)

抗原

See all Podoplanin (PDPN) ELISA试剂盒
Podoplanin (PDPN)

适用

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小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

0.15-10 ng/mL

应用范围

ELISA
  • 最低检测浓度

    0.15 ng/mL

    灵敏度

    < 0.055 ng/mL
  • 板类型

    Pre-coated

    实验流程

    Podoplanin (PDPN) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Podoplanin (PDPN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Podoplanin (PDPN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Podoplanin (PDPN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Podoplanin (PDPN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

    限制

    仅限研究用
  • 储存条件

    4 °C/-20 °C
  • 抗原 See all Podoplanin (PDPN) ELISA试剂盒

    Podoplanin (PDPN)

    途径

    Dicarboxylic Acid Transport
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