PLA2G12B ELISA 试剂盒
PLA2G12B
适用: 人
Colorimetric
Sandwich ELISA
0.15-10 ng/mL
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- 抗原 See all PLA2G12B ELISA试剂盒
- PLA2G12B (Phospholipase A2, Group XIIB (PLA2G12B))
- 适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.15-10 ng/mL
- 最低检测浓度
- 0.15 ng/mL
- 应用范围
- ELISA
- 品牌
- BioAssay™
- 灵敏度
- < 0.058 ng/mL
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Phospholipase A2, Group XIIB (PLA2G12B) ELISA Kit
PLA2G12B 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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- 板类型
- Pre-coated
- 实验流程
- Phospholipase A2, Group XIIB (PLA2G12B) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipase A2, Group XIIB (PLA2G12B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phospholipase A2, Group XIIB (PLA2G12B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phospholipase A2, Group XIIB (PLA2G12B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Phospholipase A2, Group XIIB (PLA2G12B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C/-20 °C
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- 抗原 See all PLA2G12B ELISA试剂盒
- PLA2G12B (Phospholipase A2, Group XIIB (PLA2G12B))
- Abstract
- PLA2G12B 产品
- 别名
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