PLA2G3 ELISA 试剂盒
This 小鼠 PLA2G3 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 小鼠 PLA2G3.
Quick Overview for PLA2G3 ELISA 试剂盒 (ABIN2044217)
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See all PLA2G3 ELISA试剂盒
PLA2G3
(Phospholipase A2, Group III (PLA2G3))
适用
小鼠
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
0.78-50 ng/mL
应用范围
ELISA
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最低检测浓度
- 0.78 ng/mL
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灵敏度
- < 0.27 ng/mL
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板类型
- Pre-coated
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实验流程
- Phospholipase A2, Group III (PLA2G3) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipase A2, Group III (PLA2G3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phospholipase A2, Group III (PLA2G3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phospholipase A2, Group III (PLA2G3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Phospholipase A2, Group III (PLA2G3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- PLA2G3 (Phospholipase A2, Group III (PLA2G3))
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