PDE1C ELISA 试剂盒
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Quick Overview for PDE1C ELISA 试剂盒 (ABIN2044215)
抗原
See all PDE1C ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 0.15 ng/mL
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灵敏度
- < 0.053 ng/mL
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phosphodiesterase 1C, Calmodulin-Dependent 70kDa (PDE1C) ELISA Kit
PDE1C 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
phosphodiesterase 1C, Calmodulin-Dependent 70kDa (PDE1C) ELISA KitPDE1C 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- Phosphodiesterase 1C, Calmodulin Dependent (PDE1C) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphodiesterase 1C, Calmodulin Dependent (PDE1C). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phosphodiesterase 1C, Calmodulin Dependent (PDE1C). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphodiesterase 1C, Calmodulin Dependent (PDE1C), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Phosphodiesterase 1C, Calmodulin Dependent (PDE1C) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- PDE1C (phosphodiesterase 1C, Calmodulin-Dependent 70kDa (PDE1C))
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别名
- Phosphodiesterase 1C, Calmodulin Dependent (PDE1C)
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途径
- EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Negative Regulation of Hormone Secretion, cAMP Metabolic Process, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma
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