PDE1A ELISA 试剂盒
This 人 PDE1A ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 PDE1A.
Quick Overview for PDE1A ELISA 试剂盒 (ABIN2044213)
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PDE1A
(Phosphodiesterase 1A (PDE1A))
适用
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
0.31-20 ng/mL
应用范围
ELISA
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最低检测浓度
- 0.31 ng/mL
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灵敏度
- < 0.126 ng/mL
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Phosphodiesterase 1A (PDE1A) ELISA Kit
PDE1A 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- Phosphodiesterase 1A, Calmodulin Dependent (PDE1A) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphodiesterase 1A, Calmodulin Dependent (PDE1A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phosphodiesterase 1A, Calmodulin Dependent (PDE1A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphodiesterase 1A, Calmodulin Dependent (PDE1A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Phosphodiesterase 1A, Calmodulin Dependent (PDE1A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- PDE1A (Phosphodiesterase 1A (PDE1A))
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别名
- Phosphodiesterase 1A, Calmodulin Dependent (PDE1A)
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途径
- Neurotrophin Signaling Pathway, cAMP Metabolic Process, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma
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