PER1 ELISA 试剂盒
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Quick Overview for PER1 ELISA 试剂盒 (ABIN2044195)
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检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 0.31 ng/mL
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灵敏度
- < 0.114 ng/mL
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Period Homolog 1 (Drosophila) (PER1) ELISA Kit
PER1 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Period Homolog 1 (Drosophila) (PER1) ELISA KitPER1 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- Period Circadian Protein 1 (PER1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Period Circadian Protein 1 (PER1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Period Circadian Protein 1 (PER1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Period Circadian Protein 1 (PER1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Period Circadian Protein 1 (PER1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- PER1 (Period Homolog 1 (Drosophila) (PER1))
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别名
- Period Circadian Protein 1 (PER1)
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途径
- Photoperiodism
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