KIAA0101 ELISA 试剂盒
This 人 KIAA0101 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 KIAA0101.
Quick Overview for KIAA0101 ELISA 试剂盒 (ABIN2044179)
抗原
See all KIAA0101 (PAF) ELISA试剂盒
KIAA0101 (PAF)
(PCNA Clamp Associated Factor (PAF))
适用
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
0.78-50 ng/mL
应用范围
ELISA
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最低检测浓度
- 0.78 ng/mL
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灵敏度
- < 0.27 ng/mL
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PCNA Clamp Associated Factor (PAF) ELISA Kit
PAF 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- PCNA Associated Factor 15kDa (PAF) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PCNA Associated Factor 15kDa (PAF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to PCNA Associated Factor 15kDa (PAF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PCNA Associated Factor 15kDa (PAF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of PCNA Associated Factor 15kDa (PAF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- KIAA0101 (PAF) (PCNA Clamp Associated Factor (PAF))
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别名
- PCNA Associated Factor 15kDa (PAF)
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途径
- Chromatin Binding
抗原 See all KIAA0101 (PAF) ELISA试剂盒
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