PNPLA3 ELISA 试剂盒
This 大鼠 PNPLA3 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 大鼠 PNPLA3.
Quick Overview for PNPLA3 ELISA 试剂盒 (ABIN2044177)
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PNPLA3
(Patatin-Like phospholipase Domain Containing 3 (PNPLA3))
适用
大鼠
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
0.15-10 ng/mL
应用范围
ELISA
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最低检测浓度
- 0.15 ng/mL
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灵敏度
- < 0.057 ng/mL
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Patatin-Like phospholipase Domain Containing 3 (PNPLA3) ELISA Kit
PNPLA3 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- Patatin Like Phospholipase Domain Containing Protein 3 (PNPLA3) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Patatin Like Phospholipase Domain Containing Protein 3 (PNPLA3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Patatin Like Phospholipase Domain Containing Protein 3 (PNPLA3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Patatin Like Phospholipase Domain Containing Protein 3 (PNPLA3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Patatin Like Phospholipase Domain Containing Protein 3 (PNPLA3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- PNPLA3 (Patatin-Like phospholipase Domain Containing 3 (PNPLA3))
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别名
- Patatin Like Phospholipase Domain Containing Protein 3 (PNPLA3)
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