NOSIP ELISA 试剂盒
This 人 NOSIP ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 NOSIP.
Quick Overview for NOSIP ELISA 试剂盒 (ABIN2044101)
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NOSIP
(Nitric Oxide Synthase Interacting Protein (NOSIP))
适用
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
0.15-10 ng/mL
应用范围
ELISA
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最低检测浓度
- 0.15 ng/mL
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品牌
- BioAssay™
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灵敏度
- < 0.053 ng/mL
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Nitric Oxide Synthase Interacting Protein (NOSIP) ELISA Kit
NOSIP 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- Nitric Oxide Synthase Interacting Protein (NOSIP) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nitric Oxide Synthase Interacting Protein (NOSIP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nitric Oxide Synthase Interacting Protein (NOSIP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nitric Oxide Synthase Interacting Protein (NOSIP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Nitric Oxide Synthase Interacting Protein (NOSIP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- NOSIP (Nitric Oxide Synthase Interacting Protein (NOSIP))
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