NTSR1 ELISA 试剂盒
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Quick Overview for NTSR1 ELISA 试剂盒 (ABIN2044085)
抗原
See all NTSR1 products适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 0.15 ng/mL
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灵敏度
- < 0.066 ng/mL
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板类型
- Pre-coated
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实验流程
- Neurotensin Receptor 1, High Affinity (NTSR1) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neurotensin Receptor 1, High Affinity (NTSR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neurotensin Receptor 1, High Affinity (NTSR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neurotensin Receptor 1, High Affinity (NTSR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Neurotensin Receptor 1, High Affinity (NTSR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- NTSR1 (Neurotensin Receptor 1 (High Affinity) (NTSR1))
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别名
- Neurotensin Receptor 1, High Affinity (NTSR1)
抗原 See all NTSR1 products
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