NSF ELISA 试剂盒
NSF
适用: 人
Colorimetric
Sandwich ELISA
0.31-20 ng/mL
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- 抗原 See all NSF ELISA试剂盒
- NSF (N-Ethylmaleimide-Sensitive Factor (NSF))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.31-20 ng/mL
- 最低检测浓度
- 0.31 ng/mL
- 应用范围
- ELISA
- 品牌
- BioAssay™
- 灵敏度
- < 0.116 ng/mL
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N-Ethylmaleimide-Sensitive Factor (NSF) ELISA Kit
NSF 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
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- 板类型
- Pre-coated
- 实验流程
- N-Ethylmaleimide Sensitive Factor (NSF) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to N-Ethylmaleimide Sensitive Factor (NSF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to N-Ethylmaleimide Sensitive Factor (NSF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain N-Ethylmaleimide Sensitive Factor (NSF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of N-Ethylmaleimide Sensitive Factor (NSF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C/-20 °C
-
- 抗原 See all NSF ELISA试剂盒
- NSF (N-Ethylmaleimide-Sensitive Factor (NSF))
- 别名
- N-Ethylmaleimide Sensitive Factor (NSF) (NSF 产品)
- 别名
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