MYLK ELISA 试剂盒
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Quick Overview for MYLK ELISA 试剂盒 (ABIN2044015)
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See all MYLK ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 0.15 ng/mL
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灵敏度
- < 0.057 ng/mL
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组件
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A: Microtiter Plate, 1x96 wells, Pre-coated, ready to use.
B: Standard, 2x1vial
C: Standard Diluent, 1x20 mL
D: Detection Reagent A, 1x120 µL
E: Detection Reagent B, 1x120 µL
F: Assay Diluent A, 1x10 mL
G: Assay Diluent B, 1x10 mL
H: TMB Substrate, 1x0 mL
K: Stop Solution, 1x0 mL
L: Wash Buffer, 30x, 1x20 mL
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Myosin Light Chain Kinase (MYLK) ELISA Kit
MYLK 适用: 小鼠 Colorimetric Sandwich ELISA 23.438 pg/mL - 1500 pg/mL Plasma, Serum, Tissue Homogenate
Myosin Light Chain Kinase (MYLK) ELISA KitMYLK 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Myosin Light Chain Kinase (MYLK) ELISA KitMYLK 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Myosin Light Chain Kinase (MYLK) ELISA KitMYLK 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- The Rat Myosin Light Chain Kinase (MYLK) ELISA Kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of MYLK in rat tissue homogenates and other biological fluids.The microtiter plate provided in this kit has been pre-coated with an antibody specific to MYLK. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to MYLK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MYLK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MYLK in the sample is then determined by comparing the O.D. of the sample to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- MYLK (Myosin Light Chain Kinase (MYLK))
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