MYL3/CMLC1 ELISA 试剂盒
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Quick Overview for MYL3/CMLC1 ELISA 试剂盒 (ABIN2044013)
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See all MYL3/CMLC1 (MYL3) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 78 pg/mL
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Analytical Method
- Quantitative
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灵敏度
- < 28 pg/mL
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组件
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A: Microtiter Plate, 1x96 wells, Pre-coated, ready to use
B: Standard, 2x1vial
C: Standard Diluent, 1x20 mL
D: Detection Reagent A, 1x120 µL
E: Detection Reagent B, 1x120 µL
F: Assay Diluent A, 1x10 mL
G: Assay Diluent B, 1x10 mL
H: TMB Substrate, 1x0 mL
K: Stop Solution, 1x0 mL
L: Wash Buffer, 30x, 1x20 mL
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Myosin, Light Chain 3 (MYL3) ELISA Kit
MYL3 适用: 小鼠 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Myosin, Light Chain 3 (MYL3) ELISA KitMYL3 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Myosin, Light Chain 3 (MYL3) ELISA KitMYL3 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- The Human Myosin Light Chain 3, Alkali, Ventricular, Slow Skeletal (MYL3) ELISA Kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of MYL3 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.The microtiter plate provided in this kit has been pre-coated with an antibody specific to MYL3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to MYL3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MYL3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MYL3 in the sample is then determined by comparing the O.D. of the sample to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- MYL3/CMLC1 (MYL3) (Myosin, Light Chain 3 (MYL3))
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别名
- Myosin Light Chain 3, Alkali, Ventricular, Slow Skeletal (MYL3)
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