MTAP ELISA 试剂盒
This 小鼠 MTAP ELISA Kit is a Colorimetric ELISA Kit designed to quantify 小鼠 MTAP.
Quick Overview for MTAP ELISA 试剂盒 (ABIN2043971)
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See all MTAP ELISA试剂盒
MTAP
(Methylthioadenosine phosphorylase (MTAP))
适用
小鼠
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
0.15-10 ng/mL
应用范围
ELISA
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最低检测浓度
- 0.15 ng/mL
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灵敏度
- < 0.053 ng/mL
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Methylthioadenosine phosphorylase (MTAP) ELISA Kit
MTAP 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Methylthioadenosine phosphorylase (MTAP) ELISA KitMTAP 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- Methylthioadenosine Phosphorylase (MTAP) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Methylthioadenosine Phosphorylase (MTAP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Methylthioadenosine Phosphorylase (MTAP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Methylthioadenosine Phosphorylase (MTAP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Methylthioadenosine Phosphorylase (MTAP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- MTAP (Methylthioadenosine phosphorylase (MTAP))
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途径
- Ribonucleoside Biosynthetic Process, Methionine Biosynthetic Process
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