MAN2B1 ELISA 试剂盒
This 人 MAN2B1 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 MAN2B1.
Quick Overview for MAN2B1 ELISA 试剂盒 (ABIN2043931)
抗原
See all MAN2B1 products
MAN2B1
(Mannosidase, Alpha, Class 2B, Member 1 (MAN2B1))
适用
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
31.25-2000 pg/mL
应用范围
ELISA
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最低检测浓度
- 31.25 pg/mL
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灵敏度
- < 14.7 pg/mL
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板类型
- Pre-coated
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实验流程
- Mannosidase Alpha Class 2B Member 1 (MAN2B1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mannosidase Alpha Class 2B Member 1 (MAN2B1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mannosidase Alpha Class 2B Member 1 (MAN2B1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mannosidase Alpha Class 2B Member 1 (MAN2B1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Mannosidase Alpha Class 2B Member 1 (MAN2B1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- MAN2B1 (Mannosidase, Alpha, Class 2B, Member 1 (MAN2B1))
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别名
- Mannosidase alpha Class 2B Member 1 (MAN2B1)
抗原 See all MAN2B1 products
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