MDH2 ELISA 试剂盒
This 人 MDH2 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 MDH2.
Quick Overview for MDH2 ELISA 试剂盒 (ABIN2043923)
抗原
See all MDH2 ELISA试剂盒
MDH2
(Malate Dehydrogenase 2, NAD (Mitochondrial) (MDH2))
适用
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
0.78-50 ng/mL
应用范围
ELISA
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最低检测浓度
- 0.78 ng/mL
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灵敏度
- < 0.28 ng/mL
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Malate Dehydrogenase 2, NAD (Mitochondrial) (MDH2) ELISA Kit
MDH2 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- Malate Dehydrogenase 2 (MDH2) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Malate Dehydrogenase 2 (MDH2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Malate Dehydrogenase 2 (MDH2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Malate Dehydrogenase 2 (MDH2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Malate Dehydrogenase 2 (MDH2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- MDH2 (Malate Dehydrogenase 2, NAD (Mitochondrial) (MDH2))
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别名
- Malate Dehydrogenase 2 (MDH2)
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