Lipase A ELISA 试剂盒
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- 抗原 See all Lipase A (LIPA) ELISA试剂盒
- Lipase A (LIPA) (Lipase A, Lysosomal Acid, Cholesterol Esterase (LIPA))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.31-20 ng/mL
- 最低检测浓度
- 0.31 ng/mL
- 应用范围
- ELISA
- 品牌
- BioAssay™
- 灵敏度
- < 0.117 ng/mL
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- 板类型
- Pre-coated
- 实验流程
- Lipase A, Lysosomal Acid (LIPA) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lipase A, Lysosomal Acid (LIPA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lipase A, Lysosomal Acid (LIPA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lipase A, Lysosomal Acid (LIPA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Lipase A, Lysosomal Acid (LIPA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C/-20 °C
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- 抗原 See all Lipase A (LIPA) ELISA试剂盒
- Lipase A (LIPA) (Lipase A, Lysosomal Acid, Cholesterol Esterase (LIPA))
- 别名
- Lipase A, Lysosomal Acid (LIPA) (LIPA 产品)
- 别名
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