Lipase A ELISA 试剂盒
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Quick Overview for Lipase A ELISA 试剂盒 (ABIN2043893)
抗原
See all Lipase A (LIPA) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 0.31 ng/mL
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灵敏度
- < 0.117 ng/mL
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Lipase A, Lysosomal Acid, Cholesterol Esterase (LIPA) ELISA Kit
LIPA 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Lipase A, Lysosomal Acid, Cholesterol Esterase (LIPA) ELISA KitLIPA 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- Lipase A, Lysosomal Acid (LIPA) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lipase A, Lysosomal Acid (LIPA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lipase A, Lysosomal Acid (LIPA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lipase A, Lysosomal Acid (LIPA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Lipase A, Lysosomal Acid (LIPA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- Lipase A (LIPA) (Lipase A, Lysosomal Acid, Cholesterol Esterase (LIPA))
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别名
- Lipase A, Lysosomal Acid (LIPA)
抗原 See all Lipase A (LIPA) ELISA试剂盒
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