LIFR ELISA 试剂盒
Quick Overview for LIFR ELISA 试剂盒 (ABIN2043887)
抗原
See all LIFR ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 0.31 ng/mL
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品牌
- BioAssay™
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灵敏度
- < 0.113 ng/mL
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Leukemia Inhibitory Factor Receptor alpha (LIFR) ELISA Kit
LIFR 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Leukemia Inhibitory Factor Receptor alpha (LIFR) ELISA KitLIFR 适用: 大鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Leukemia Inhibitory Factor Receptor alpha (LIFR) ELISA KitLIFR 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Leukemia Inhibitory Factor Receptor alpha (LIFR) ELISA KitLIFR 适用: 人 AA 45-833 Colorimetric Sandwich ELISA 156-10.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
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板类型
- Pre-coated
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实验流程
- Leukemia Inhibitory Factor Receptor (LIFR) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Leukemia Inhibitory Factor Receptor (LIFR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Leukemia Inhibitory Factor Receptor (LIFR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Leukemia Inhibitory Factor Receptor (LIFR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Leukemia Inhibitory Factor Receptor (LIFR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- LIFR (Leukemia Inhibitory Factor Receptor alpha (LIFR))
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别名
- Leukemia Inhibitory Factor Receptor (LIFR)
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途径
- JAK/STAT Signaling, Growth Factor Binding
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